Crystal structure of GerE, the ultimate transcriptional regulator of spore formation in Bacillus subtilis
Crystal structure of GerE, the ultimate transcriptional regulator of spore formation in Bacillus subtilis
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DOI：
10.1006/jmbi.2001.4443
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发表时间：
2001-03-02
影响因子：
5.6
通讯作者：
Brannigan, JA
中科院分区：
文献类型：
--
作者：
Ducros, VMA;Lewis, RJ;Brannigan, JA
小的DNA结合蛋白GerE在枯草芽孢杆菌孢子形成的后期调节终末分化的母细胞区室中的基因转录。这种多功能转录因子与LuxR/FixJ/UhpA家族的激活因子具有序列同源性，并调节许多基因的表达，特别是那些编码成熟孢子周围外壳组分的基因。GerE协调外壳沉积和成熟的最后阶段，导致孢子具有显着的抗性特性，但必须响应低水平的萌发信号。由于这种萌发过程在很大程度上是被动的，并且可以在没有de Move蛋白合成的情况下发生，因此萌发机制的正确组装，包括萌发受体和能量储存化合物，对细胞的存活至关重要。Ge E的晶体结构已经解决了在2.05埃分辨率使用多波长反常色散技术，揭示了Ge E二聚体的性质。每个单体包含四个α-螺旋，其中中心对形成螺旋-转角-螺旋DNA结合基序。DNA结合和LuxR/FixJ/UhpA家族的转录激活因子结构域的结构组织的影响进行了讨论。(C)北京：科学出版社.
The small, DNA-binding protein GerE regulates gene transcription in the terminally differentiated mother-cell compartment during late stages of sporulation in Bacillus subtilis. This versatile transcription factor shares sequence homology with the LuxR/FixJ/UhpA family of activators and modulates the expression of a number of genes, in particular those encoding the components of the coat that surrounds the mature spore. GerE orchestrates the final stages of coat deposition and maturation that lead to a spore with remarkable resistance properties but that must be responsive to low levels of germination signals. As this germination process is largely passive and can occur in the absence of de Move protein synthesis, the correct assembly of germination machinery, including germinant receptors and energy storage compounds, is crucial to the survival of the cell. The crystal structure of GerE has been solved at 2.05 Angstrom resolution using multi-wavelength anomalous dispersion techniques and reveals the nature of the GerE dimer. Each monomer comprises four a-helices, of which the central pair forms a helix-turn-helix DNA-binding motif. Implications for DNA-binding and the structural organisation of the LuxR/FixJ/UhpA family of transcription activator domains are discussed. (C) 2001 Academic Press.