The role of recombination and mutation in 16S-23S rDNA spacer rearrangements
The role of recombination and mutation in 16S-23S rDNA spacer rearrangements
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DOI：
10.1016/s0378-1119(99)00224-3
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发表时间：
1999-09-30
期刊：
影响因子：
3.5
通讯作者：
Gürtler, V
中科院分区：
文献类型：
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作者：
Gürtler, V
对细菌基因间隔区(16S-23S rDNA)间隔区(ISR)的基因组内异质性进行了分析，这些物种包括：粪肠球菌(6)和粪肠球菌(6)，枯草杆菌(10)，金黄色葡萄球菌(9)，霍乱弧菌(4)，流感嗜血杆菌(6)和大肠杆菌(7)。研究发现，基因组操纵子之间的一些间隔区序列是高度保守的，而其他间隔区序列块的存在是可变的。当使用程序Plato和由DNAml为每个操纵子集合确定的偏似然系统发生分析这些变异时，三个区域表现出显著的(Z&>3.3)空间变异[区域I是78-184个核苷酸长(2.1
The intragenomic heterogeneity of the bacterial intergenic (16S-23S rDNA) spacer region (ISR) was analysed from the following species in which sequences for the complete rRNA operon (rrn) set have been determined (rrn number): Enterococcus faecalis (6) and E. faecium (6), Bacillus subtilis (10), Staphylococcus aureus (9), Vibrio cholerae (4), Haemophilus influenzae (6) and Escherichia coli (7). It was found that some spacer sequence blocks were highly conserved between operons of a genome, whereas the presence of others was variable. When these variations were analysed using the program PLATO and partial likelihood phylogenies determined by DNAml for each operon set, three regions showed significant (Z>3.3) spatial variation [Region I was 78-184 nt long (2.1