Dinucleotide repeat polymorphism at the GABAA receptor beta 3 (GABRB3) locus in the Angelman/Prader-Willi region (AS/PWS) of chromosome 15.
Dinucleotide repeat polymorphism at the GABAA receptor beta 3 (GABRB3) locus in the Angelman/Prader-Willi region (AS/PWS) of chromosome 15.
复制标题
15 号染色体 Angelman/Prader-Willi 区域 (AS/PWS) 的 GABAA 受体 β 3 (GABRB3) 位点的二核苷酸重复多态性。
DOI：
10.1093/hmg/1.1.67
复制
发表时间：
1992
影响因子：
3.5
通讯作者：
Ledbetter,DH
中科院分区：
文献类型：
--
作者：
Mutirangura,A;Ledbetter,SA;Kuwano,A;Chinault,AC;Ledbetter,DH
Source/Description: The GABRB3 gene has previously been mapped to proximal 15q in the AS/PWS region (1). Using primers generated from the sequence of the rat GABRB3 cDNA (2), a DNA probe was generated by PCR of human genomic DNA and used to screen a total human YAC libary (3). Clone B25E9 (150 kb) was identified and confirmed to map to proximal 15q by fluorescence in situ hybridization. Alu-PCR products from this YAC were screened for CA repeats by modification of a PCR method using a combination of Alu and CA or GT primers (4). Positive fragments were subcloned into pBS SK and sequenced; sequences flanking a ACAC (CA) 17 repeat element were used to design PCR primers.(EMBL accession no. X63670.)
