Curcumin protects against Aflatoxin B1-induced liver injury in broilers via the modulation of long non-coding RNA expression
Curcumin protects against Aflatoxin B1-induced liver injury in broilers via the modulation of long non-coding RNA expression
复制标题
DOI：
10.1016/j.ecoenv.2020.111725
复制
发表时间：
2021-01-15
影响因子：
6.8
通讯作者：
Zhang, Xiuying
中科院分区：
文献类型：
--
作者：
Li, Sihong;Liu, Ruimeng;Zhang, Xiuying
黄曲霉毒素 B1 (AFB1) 是一种强效肝毒性和致癌剂。姜黄素具有潜在的抗炎、抗氧化和保肝作用。然而，LncRNA 在姜黄素对抗 AFB1 诱导的肝损伤的保护机制中的作用仍不清楚。实验肉鸡被随机分为1）对照组，2）AFB1组（1 mg/kg饲料），3）cur + AFB1组（1 mg/kg AFB1加300 mg/kg姜黄素日粮）和4）姜黄素组（300 mg/kg姜黄素日粮）。进行肝脏转录组分析和 qPCR 来鉴定基因表达的变化。此外，还确定了组织病理学评估和氧化状态。膳食AFB1引起肝脏形态损伤，显着增加ROS的产生，降低肝脏抗氧化酶活性并诱导炎症和细胞凋亡。然而，膳食姜黄素部分减轻了肝组织的异常形态变化、氧化应激和细胞凋亡。转录谱结果显示，AFB1 和姜黄素联合处理后，肉鸡肝脏中有 34 个 LncRNA 和 717 个 mRNA 出现差异表达。 LncRNA-mRNA 网络、GO 和 KEGG 富集数据的分析表明，氧化应激、炎症和凋亡途径对于姜黄素减轻 AFB1 诱导的肝损伤至关重要。总之，姜黄素通过 LncRNA 预防 AFB1 诱导的氧化应激、炎症和细胞凋亡。这些结果为揭示姜黄素对抗 AFB1 诱导的肝损伤的保护机制提供了新的见解。
Aflatoxin B1 (AFB1) is a potent hepatotoxic and carcinogenic agent. Curcumin possesses potential anti-inflammatory, anti-oxidative and hepatoprotective effects. However, the role of LncRNAs in the protective mechanisms of curcumin against AFB1-induced liver damage is still elusive. Experimental broilers were randomly divided into 1) control group, 2) AFB1 group (1 mg/kg feed), 3) cur + AFB1 group (1 mg/kg AFB1 plus 300 mg/kg curcumin diet) and 4) curcumin group (300 mg/kg curcumin diet). Liver transcriptome analyses and qPCR were performed to identify shifts in genes expression. In addition, histopathological assessment and oxidant status were determined. Dietary AFB1 caused hepatic morphological injury, significantly increased the production of ROS, decreased liver antioxidant enzymes activities and induced inflammation and apoptosis. However, dietary curcumin partially attenuated the abnormal morphological changes, oxidative stress, and apoptosis in liver tissues. Transcriptional profiling results showed that 34 LncRNAs and 717 mRNAs were differentially expressed with AFB1 and curcumin co-treatment in livers of broilers. Analysis of the LncRNA-mRNA network, GO and KEGG enrichment data suggested that oxidative stress, inflammation and apoptosis pathway were crucial in curcumin's alleviating AFB1-induced liver damage. In conclusion, curcumin prevented AFB1-induced oxidative stress, inflammation and apoptosis through LncRNAs. These results provide new insights for unveiling the protective mechanisms of curcumin against AFB1-induced liver damage.