Exoamylase activity in vacuoles isolated from pea and wheat leaf protoplasts.

Exoamylase activity in vacuoles isolated from pea and wheat leaf protoplasts.
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从豌豆和小麦叶原生质体分离的液泡中的外切淀粉酶活性。

DOI:
10.1104/pp.82.4.1119
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发表时间:
1986
期刊:
影响因子:
7.4
通讯作者:
E. Beck
E. Beck
中科院分区:
生物学1区
文献类型:
--
作者:
P. Ziegler;E. Beck

文献摘要

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从豌豆和小麦叶原生质体中分离出的淀粉酶含有相当高的酶活性,且酶活性具有很高的移动的活性。菠菜叶片和光合自养红藜悬浮培养细胞原生质体的淀粉水解产物均不具有淀粉水解活性。在所有情况下,内淀粉酶活性主要与叶绿体。
Vacuoles isolated from pea (Pisum sativum), and wheat (Triticum aestivum) leaf protoplasts contained considerable activities of electrophoretically highly mobile exoamylases. Vacuoles from spinach (Spinacia oleracea) leaf and photoautotrophic Chenopodium rubrum suspension culture cell protoplasts were devoid of amylolytic activity. Endoamylase activity was in all cases associated primarily with the chloroplast.