Changes in Mitochondrial Respiratory Chain Components of Petunia Cells during Culture in the Presence of Antimycin A
Changes in Mitochondrial Respiratory Chain Components of Petunia Cells during Culture in the Presence of Antimycin A
复制标题
抗霉素A培养过程中矮牵牛细胞线粒体呼吸链成分的变化
DOI：
10.1104/pp.115.2.617
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
M. J. Wagner
中科院分区：
文献类型：
--
作者：
A. Wagner;M. J. Wagner
当矮牵牛（Petunia hybrida Vilm，cv Rosy Morn）细胞在2 [mu]M抗霉素A（AA）存在下培养时，呼吸主要通过抗氰化物途径进行。抗氰呼吸速率较高的线粒体从AA细胞比对照线粒体。与对照细胞相比，AA细胞中交替氧化酶蛋白的含量增加，以及泛醌（UQ）含量增加。琥珀酸脱氢酶的动力学的变化进行了观察：有一个高得多的活动在高UQ减少线粒体AA细胞与对照线粒体相比。未发现外部NADH脱氢酶动力学的变化。在体内AA细胞中，UQ减少仅略高于对照细胞，表明通过旁路途径增加的电子传递可以防止高UQ减少水平。此外，O2消耗继续以与对照细胞相似的速率进行，从而防止O2危险。这些适应压力条件下，其中细胞色素途径是有限的，显然需要，除了增加替代氧化酶蛋白，一个新的设置的相对量和/或动力学参数的呼吸网络的所有单独的组件。
When petunia (Petunia hybrida Vilm, cv Rosy Morn) cells are cultured in the presence of 2 [mu]M antimycin A (AA), respiration proceeds mainly via the cyanide-resistant pathway. Cyanide-resistant respiratory rates were higher in mitochondria from AA cells than in control mitochondria. Compared with control cells, an increase in alternative oxidase protein was observed in AA cells, as well as an increase in ubiquinone (UQ) content. A change in the kinetics of succinate dehydrogenase was observed: there was a much higher activity at high UQ reduction in mitochondria from AA cells compared with control mitochondria. No changes were found for external NADH dehydrogenase kinetics. In AA cells in vivo, UQ reduction was only slightly higher than in control cells, indicating that increased electron transport via the alternative pathway can prevent high UQ reduction levels. Moreover, O2 consumption continues at a similar rate as in control cells, preventing O2 danger. These adaptations to stress conditions, in which the cytochrome pathway is restricted, apparently require, in addition to an increase in alternative oxidase protein, a new setup of the relative amounts and/or kinetic parameters of all of the separate components of the respiratory network.