Neuroprotective effects of TongLuoJiuNao in neurons exposed to oxygen and glucose deprivation
Neuroprotective effects of TongLuoJiuNao in neurons exposed to oxygen and glucose deprivation
复制标题
DOI：
10.1016/j.jep.2012.03.042
复制
发表时间：
2012-06-14
影响因子：
5.4
通讯作者：
Hua, Qian
中科院分区：
文献类型：
--
作者：
Li, Xiao-Jing;Hou, Jin-Cai;Hua, Qian
民族药理学相关性：通络救脑（TLJN）是一种中药提取物，主要含有人参皂苷Rg 1和京尼平苷，临床上用于治疗脑缺血性损伤。本研究目的：脑卒中时，脑缺血后氧再灌注诱导海马神经元凋亡，而神经元轴突和树突的伸展可能对缺氧脑神经网络的损伤进行代偿和修复。在这项研究中，我们研究了TLJN是否可以保护神经元免受脑血管缺血损伤的材料和方法：我们测定了细胞活力和乳酸脱氢酶（LDH）释放从原代培养的大鼠海马神经元之前和之后的神经元缺氧缺糖（OGD）。结果：TLJN对体外培养的原代大鼠海马神经元在正常和氧/糖剥夺（OGD）条件下均具有神经保护作用。TLJN能保护原代培养的大鼠海马神经元和脑微血管内皮细胞（BMEC）免受正常和氧/糖剥夺（OGD）条件下的细胞死亡。结论：TLJN具有明显的神经保护和神经营养作用，其作用机制可能与TLJN的作用机制有关。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: TongLuoJiuNao (TLJN) is an herb extract that mainly contains ginsenoside Rg1 and geniposide, which are clinically used for treating ischemic damages in the brain.Aim of the study: In the stroke, cerebral ischemia followed by oxygen reperfusion induced apoptosis in hippocampal neurons, while extension of axons and dendrites in neurons may compensate for and repair damages of neuronal network in the hypoxia brain. In this study, we investigated whether TLJN can protect neurons against damages by ischemia in brain vasculature.Materials and methods: We measured cell viability and lactate dehydrogenase (LDH) release from primary culture of rat hippocampal neurons before and after the neurons were deprived of oxygen and glucose (OGD). In addition, the effects were evaluated with cell viability and neurite outgrowth before or after OGD.Results: We found that TLJN could play a neuroprotective role to cultured primary rat hippocampal neurons under both normal and oxygen/glucose-deprivation (OGD) conditions. TLJN could protect both cultured primary rat hippocampal neurons and brain microvascular endothelial cells (BMECs) from cell death under both normal and oxygen/glucose-deprivation (OGD) conditions. Moreover, under the same conditions, BMECs-conditioned media pretreated by TNJN could also promote neuron viability and neurite outgrowth, indicating that TUN stimulated BMECs to secret some neuroprotective/neurotrophic factors.Conclusion: These findings suggest that TLJN has a marked neuroprotective and neurotrophic roles by either direct or indirect operation, and provide insight into the mechanism of clinical efficacy of this drug against stroke. (C) 2012 Elsevier Ireland Ltd. All rights reserved.