Direct template matching reveals a host subcellular membrane gyroid cubic structure that is associated with SARS virus
Direct template matching reveals a host subcellular membrane gyroid cubic structure that is associated with SARS virus
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DOI：
10.1179/135100005x57373
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发表时间：
2005-06-01
期刊：
影响因子：
3.8
通讯作者：
Deng, Y
中科院分区：
文献类型：
--
作者：
Almsherqi, ZA;McLachlan, CS;Deng, Y
病毒感染可导致宿主亚细胞膜的改变。这是经常报告时，使用透射电子显微镜（TEM），导致在tubuloreticular膜亚细胞超微结构的描述，而不是基于三维形态的定义。描述亚细胞膜变化的2-D TEM显微照片与亚细胞SARS病毒粒子相关[金匠CS，Tatti KM，Ksiazek TG et al. Ultra-structural characterization of SARS coronavirus. Emerg Infect Dis 2004; 10：320 - 326]。在本研究中，我们已经确定了与SARS病毒感染相关的二维膜模式和形状。这是通过使用直接模板匹配方法来确定SARS病毒相关宿主膜变化的三维结构。用于我们目的的TEM图像是基于2-D信息定义的，例如膜已经经历了增殖，并且来自模式识别，这表明膜描述的模式可能是螺旋状膜。膜的特征被用来计算和匹配的螺旋结构与现有的2-D TEM显微照片，其中它被揭示的膜结构确实是一个基于螺旋的立方膜。用于匹配感兴趣的电子显微照片的二维螺旋体计算机模拟图像来自数学上定义良好的三维结构，正是从这个三维衍生物中，我们可以推断出这个膜的三维结构。总之，我们证明了一个3-D结构可以从2-D膜图案化的图像和SARS病毒相关的膜变化已被确定为立方膜形态。这种立方膜变化的可能机制进行了讨论，相对于病毒的严重程度，持久性和自由基的产生。
Viral infection can result in alterations to the host subcellular membrane. This is often reported when using transmission electron microscopy (TEM), resulting in a description of tubuloreticular membrane subcellular ultrastructure rather than a definition based on 3-D morphology. 2-D TEM micrographs depicting subcellular membrane changes are associated with subcellular SARS virion particles [ Goldsmith CS, Tatti KM, Ksiazek TG et al. Ultra-structural characterization of SARS coronavirus. Emerg Infect Dis 2004; 10: 320 - 326]. In the present study, we have defined the 2-D membrane pattern and shape associated with the SARS virus infection. This is by using a direct template matching method to determine what the 3-D structure of the SARS virus associated host membrane change would be. The TEM image for our purposes is defined on 2-D information, such as the membrane having undergone proliferation and from pattern recognition suggesting that the membrane-described pattern is possibly a gyroid type of membrane. Features of the membrane were used to compute and match the gyroid structure with an existing 2-D TEM micrograph, where it was revealed that the membrane structure was indeed a gyroid-based cubic membrane. The 2-D gyroid computer-simulated image that was used to match the electron micrograph of interest was derived from a mathematically well-defined 3-D structure, and it is from this 3-D derivative that allows us to make inferences about the 3-D structure of this membrane. In conclusion, we demonstrate that a 3-D structure can be defined from a 2-D membrane patterned image and that a SARS viral associated membrane change has been identified as cubic membrane morphology. Possible mechanisms for this cubic membrane change are discussed with respect to viral severity, persistence and free radical production.