Identification of 5S and 45S rDNA sites in Chrysanthemum species by using oligonucleotide fluorescence in situ hybridization (Oligo-FISH)
Identification of 5S and 45S rDNA sites in Chrysanthemum species by using oligonucleotide fluorescence in situ hybridization (Oligo-FISH)
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DOI：
10.1007/s11033-020-06102-1
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发表时间：
2021-01-17
影响因子：
2.8
通讯作者：
Wang, Haibin
中科院分区：
文献类型：
--
作者：
He, Jun;Lin, Sisi;Wang, Haibin
荧光原位杂交(FISH)是一种用于可视化DNA元件在基因组中的分布的传统方法。为了研究菊花属内的亲缘关系，利用核糖体DNA(RDNA)，一个流行的细胞遗传学标记，作为该属内鱼类的探针。本研究以菊科菊科菊属(菊科)中的菊花、菊花及其近缘属的基因组数据为基础，利用rDNA序列设计寡核苷酸探针，并对8份菊花材料进行寡核苷酸荧光原位杂交。结果表明，5S rDNA信号大多位于染色体亚末端区域，5S rDNA位点的数量可能与倍性密切相关。对于45S rDNA位点，信号的数量和强度与以前调查的菊花资源不同。这些发现可能为研究菊花及其近缘物种的染色体组成和结构变异提供一种最可靠的方法，并使研究人员能够了解菊花的进化史和系统发育关系。
Fluorescence in situ hybridization (FISH) is a conventional method used to visualize the distribution of DNA elements within a genome. To examine the relationships within the Chrysanthemum genus, ribosomal DNA (rDNA), a popular cytogenetic marker, was utilized as a probe for FISH within this genus. Based on the genome data of Chrysanthemum nankingense, C. seticuspe and its allied genera in the Compositae(Asteraceae), we explored rDNA sequences to design oligonucleotide probes and perform oligonucleotide fluorescence in situ hybridization (Oligo-FISH) in eight Chrysanthemum accessions. The results showed that the majority of 5S rDNA signals were located in subterminal chromosome regions and that the number of 5S rDNA sites might be tightly associated with ploidy. For 45S rDNA sites, the number and intensity of signals differed from those of previously investigated Chrysanthemum resources. These findings may provide an optimally reliable method of examining the chromosome composition and structural variation of Chrysanthemum and its related species and allow researchers to understand the evolutionary history and phylogenetic relationships of Chrysanthemum.