Reconstruction and representation of caudal vasculature of zebrafish embryo from confocal scanning laser fluorescence microscopic images
Reconstruction and representation of caudal vasculature of zebrafish embryo from confocal scanning laser fluorescence microscopic images
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DOI：
10.1016/j.compbiomed.2004.05.003
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发表时间：
2005-12-01
影响因子：
7.7
通讯作者：
Ip, HHS
中科院分区：
文献类型：
--
作者：
Feng, J;Cheng, SH;Ip, HHS
从一系列切片进行三维（3D）重建是医学成像中的一项重要技术，特别是用于血管造影的血管可视化。在这里，我们提出了一个自动分割和配准2天大斑马鱼胚胎不同类型血管的框架。将荧光微珠（0.02 μ m）标记的血管注射到2日龄斑马鱼胚胎的血流中，从共聚焦显微镜获得一系列光学切片。提取血管并计算其形态学参数，包括长度和直径。与此同时，自动登记单个血管。血管系统用属性血管表示图（AVRG）表示，AVRG包含了血管的形态学数据和连通性。使用AVRG来表示脉管系统使得不同胚胎的脉管系统之间的比较更加容易。AVRG三维重建模型的可视化和量化在交互式界面中呈现。该框架是用Visual C++作为基于Windows的程序来实现的。(c)2004爱思唯尔有限公司保留所有权利。
Three-dimensional (3D) reconstruction from a series of sections is an important technique in medical imaging, particularly for visualization of blood vessels from angiography. Here, we present a framework for automatic segmentation and registration of different kind of blood vessels from 2-day-old zebrafish embryos. Series of optical sections were acquired from confocal microscopy with the blood vessels labeled by fluorescent microbeads (0.02 mu m) injected into blood stream of 2-day-old zebrafish embryos. Blood vessels were extracted and their morphological parameters, including length and diameter, were calculated. At the same time, individual blood vessels were registered automatically. Vasculature was represented by attributed vessel represent graph (AVRG), which contained morphological data and connectivity of every blood vessel. Using AVRG to represent a vasculature made the comparison between vasculatures of different embryos more easy. Visualization, as well as quantification, of reconstructed 3D model of AVRG was presented in an interactive interface. The framework was implemented by Visual C++ as Windows-based program. (c) 2004 Elsevier Ltd. All rights reserved.