SPR study for analysis of a water-soluble glycopolymer interface and molecular recognition properties
SPR study for analysis of a water-soluble glycopolymer interface and molecular recognition properties
复制标题
DOI：
10.1038/pj.2016.99
复制
发表时间：
2017-02
期刊：
影响因子：
2.8
通讯作者：
Yuhei Terada;H. Seto;Y. Hoshino;T. Murakami;S. Shinohara;K. Tamada;Y. Miura
中科院分区：
文献类型：
--
作者：
Yuhei Terada;H. Seto;Y. Hoshino;T. Murakami;S. Shinohara;K. Tamada;Y. Miura
采用可逆加成断裂链转移（RAFT）聚合法合成了不同甘露糖掺入率（10%和100%）的甘露糖和丙烯酰胺的糖基共聚物，并在金基底上制备了聚合物膜。利用表面等离子体共振（SPR）技术分析了聚合物的层结构和分子识别性能。在空气中形成厚度为1.2 nm的薄饼状薄层，并在水溶液中溶胀。利用SPR技术分析了其对刀豆球蛋白A（ConA）的分子识别作用。glycopolymers和刀豆蛋白A之间的结合常数大到足以表明多价效应。ConA的糖共聚物的结合速率常数是在相同的顺序，然而，解离速率常数较低的糖共聚物与甘露糖比为100%，因为高的本地甘露糖密度附近的ConA的结合点。
Glycopolymers consisting of mannose and acrylamide with different mannose incorporation ratios (10 and 100%) were synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization, and the polymer layers were prepared on a gold substrate. The detailed polymer layer structure and molecular recognition properties were analyzed by the surface plasmon resonance (SPR) technique. The glycopolymers formed pancake-like thin layers with thicknesses of∼ 2 nm in air and were swollen in the aqueous solution. The molecular recognition against concanavalin A (ConA) was also analyzed by SPR. Binding constants between glycopolymers and ConA were large enough to suggest multivalent effects. Binding rate constants of ConA to glycopolymers were in the same order; however, the dissociation rate constant was lower in the glycopolymer with a mannose ratio of 100% because of the high local mannose density near the binding point of ConA.