High-Throughput Sequence-Based Analysis of the Intestinal Microbiota of Weanling Pigs Fed Genetically Modified MON810 Maize Expressing Bacillus thuringiensis Cry1Ab (Bt Maize) for 31 Days
High-Throughput Sequence-Based Analysis of the Intestinal Microbiota of Weanling Pigs Fed Genetically Modified MON810 Maize Expressing Bacillus thuringiensis Cry1Ab (Bt Maize) for 31 Days
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DOI：
10.1128/aem.00307-12
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发表时间：
2012-06-01
影响因子：
4.4
通讯作者：
Lawlor, Peadar G.
中科院分区：
文献类型：
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作者：
Buzoianu, Stefan G.;Walsh, Maria C.;Lawlor, Peadar G.
本研究的目的是研究饲喂表达苏云金芽孢杆菌杀虫蛋白(Bt玉米)的转基因MON810玉米对猪肠道微生物区系的影响。28日龄断奶的18头猪，经过6天的驯化后，被分配到含有转基因(Bt MON810)玉米或非转基因等基因亲本玉米的日粮中31天(n=9/处理)。通过依赖培养和独立培养的方法来评估对猪肠道微生物区系的影响。饲喂等基因日粮和Bt玉米日粮的猪的粪便、盲肠和愈合的总厌氧菌、肠杆菌科和乳杆菌的数量没有显著差异。此外，高通量16S rRNA基因测序显示，盲肠微生物群的组成几乎没有差异。唯一不同的是，饲喂Bt玉米日粮的猪盲肠中肠球菌科(0.06比0%；P<；0.05)、白刺球菌科(1.28比1.17%；P<；0.05)和双歧杆菌(0.04比0%；P<；0.05)的盲肠细菌丰度高于等基因玉米日粮猪(0.23比0.40%；P<；0.05)。Bt玉米中较低的抗酶淀粉含量，很可能是正常变异的结果，而不是遗传修饰的结果，这可能解释了盲肠微生物群中观察到的一些差异。这些结果表明，转Bt基因玉米对猪肠道微生物区系具有良好的耐受性，为转Bt基因玉米的安全性评价提供了补充数据。此外，考虑到猪作为人类模型的适用性，这些数据可能会外推到人类身上。
The objective of this study was to investigate if feeding genetically modified (GM) MON810 maize expressing the Bacillus thuringiensis insecticidal protein (Bt maize) had any effects on the porcine intestinal microbiota. Eighteen pigs were weaned at 28 days and, following a 6-day acclimatization period, were assigned to diets containing either GM (Bt MON810) maize or non-GM isogenic parent line maize for 31 days (n = 9/treatment). Effects on the porcine intestinal microbiota were assessed through culture-dependent and -independent approaches. Fecal, cecal, and heal counts of total anaerobes, Enterobacteriaceae, and Lactobacillus were not significantly different between pigs fed the isogenic or Bt maize-based diets. Furthermore, high-throughput 16S rRNA gene sequencing revealed few differences in the compositions of the cecal microbiotas. The only differences were that pigs fed the Bt maize diet had higher cecal abundance of Enterococcaceae (0.06 versus 0%; P < 0.05), Erysipelotrichaceae (1.28 versus 1.17%; P < 0.05), and Bifidobacterium (0.04 versus 0%; P < 0.05) and lower abundance of Blautia (0.23 versus 0.40%; P < 0.05) than pigs fed the isogenic maize diet. A lower enzyme-resistant starch content in the Bt maize, which is most likely a result of normal variation and not due to the genetic modification, may account for some of the differences observed within the cecal microbiotas. These results indicate that Bt maize is well tolerated by the porcine intestinal microbiota and provide additional data for safety assessment of Bt maize. Furthermore, these data can potentially be extrapolated to humans, considering the suitability of pigs as a human model.