Anti-Trypanosoma brucei activity of nonprimate zoo Sera
Anti-Trypanosoma brucei activity of nonprimate zoo Sera
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DOI：
10.2307/3285698
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发表时间：
1999-02-01
影响因子：
1.3
通讯作者：
Seed, JR
中科院分区：
文献类型：
--
作者：
Black, SJ;Wang, Q;Seed, JR
组成型抗布氏锥虫亚种在来自22种非灵长类哺乳动物的血清中评价了布鲁氏菌S 427克隆1和22的活性。血清分为5类。来自好望角布法罗、长颈鹿和大捻角羚的血清对这2个克隆的生物体的复制表现出浓度依赖性抑制，这依赖于黄嘌呤氧化酶的存在。血清从疣猪和跳羚也严重限制锥虫复制，但缺乏黄嘌呤氧化酶。它们的抗锥虫活性在56 ℃加热30分钟后被灭活，但在4 ℃下被锥虫吸收后不受影响。狮、豹血清对T.布氏S427克隆1株，未克隆22株。这些血清缺乏黄嘌呤氧化酶。他们的抗T通过在56 ℃加热30分钟使布氏S427克隆1活性失活，但不能通过用锥虫吸收而除去。格兰特瞪羚的血清阻止了T。布氏克隆缺乏黄嘌呤氧化酶，并且不受56 ℃加热的影响。从水羚，汤普森的瞪羚，sitatunga，角hartebeeste，gerenuk，格兰特斑马，牛，薮猫，美洲狮，山猫，家猫的血清是完全支持锥虫复制，无论测试浓度高达48%v/v的最大培养基。来自相同哺乳动物物种的不同个体的血清对锥虫具有相似的作用，并且在不同时间从相同个体收集的样品也具有相似的活性，表明组成型血清抗锥虫组分的物种特异性稳定表达或缺乏。
Constitutive anti-Trypanosoma brucei subsp. brucei S 427 clone 1 and 22 activities were evaluated in sera from 22 species of nonprimate mammals. The sera fell into 5 categories. Sera from Cape buffalo, giraffe, and greater kudu showed a concentration-dependent inhibition of replication of the 2 clones of organisms, which was dependent on the presence of xanthine oxidase. Sera from warthog and springbok also severely limited trypanosome replication but lacked xanthine oxidase. Their antitrypanosome activity was inactivated by heating at 56 C for 30 min but not affected by absorbing with trypanosomes at 4 C. Sera from lion and leopard showed a concentration-dependent inhibition of the growth of T. brucei S427 clone 1 organisms, but not clone 22 organisms. These sera lacked xanthine oxidase. Their anti-T. brucei S 427 clone 1 activity was inactivated by heating at 56 C for 30 min bur not removed by absorbing with trypanosomes. Serum from Grant's gazelle prevented replication of both T. brucei clones, lacked xanthine oxidase, and was not affected by heating at 56 C. Sera from waterbuck, Thompson's gazelle, sitatunga, Cape hartebeeste, gerenuk, Grant's zebra, cow, serval cat, cougar, bobcat, and domestic cat were fully supportive of trypanosome replication irrespective of concentration tested up to a maximum of 48% v/v in culture medium. Sera from different individuals of the same mammal species had similar effects on trypanosomes, and samples collected from the same individual at different times also had similar activities indicating species-specific stable expression, or lack thereof, of constitutive serum antitrypanosome components.