Succession of microbial community and enhanced mechanism of a ZVI-based anaerobic granular sludge process treating chloronitrobenzenes wastewater.
Succession of microbial community and enhanced mechanism of a ZVI-based anaerobic granular sludge process treating chloronitrobenzenes wastewater.
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DOI：
10.1016/j.jhazmat.2014.11.029
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发表时间：
2015-03
影响因子：
13.6
通讯作者：
Liang Zhu;Jie Jin;Hai-zhuan Lin;K. Gao;Xiang-yang Xu
中科院分区：
文献类型：
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作者：
Liang Zhu;Jie Jin;Hai-zhuan Lin;K. Gao;Xiang-yang Xu
建立了零价铁（ZVI）-升流式厌氧污泥床（UASB）组合工艺处理氯代硝基苯（ClNBs）废水，研究了该工艺中微生物群落的演替及其强化机理。结果表明，在进水COD和3，4-二氯硝基苯的条件下，与对照UASB（R1）相比，组合系统（R2）对COD的去除、ClNBs的转化和脱氯效果稳定（3，4-DClNB）负载率为4200-7700 g m-3d-1和6.0-70.0 g m-3d-1，R1和R2具有较好的抗冲击能力和缓冲厌氧酸化能力。对氯苯胺（p-ClAn）中间产物在R2反应器中45 d后发生脱氯反应生成苯胺（AN），而R1反应器在长期运行后未发生脱氯反应。在该组合系统中成功开发了新型ZVI基厌氧颗粒污泥（ZVI-AGS），并同时实现了较高的微生物活性，包括ClNB转化和H2/CH 4生产。优势菌群与Megaspaera、Chloroflexi和Clostridium密切相关，绝大多数古菌群与Methanosaetaconcilii和Methanothrixsoehngenii相关，它们在厌氧生态位和EPS分泌中具有还原脱氯PCB、HCB和TCE的能力。
The combined zero-valent iron (ZVI) and upflow anaerobic sludge blanket (UASB) process is established for the treatment of chloronitrobenzenes (ClNBs) wastewater, and the succession of microbial community and its enhanced mechanism are investigated in the study. Results showed that compared with the control UASB (R1), the stable COD removal, ClNBs transformation, and dechlorination occurred in the combined system (R2) when operated at influent COD and 3,4-Dichloronitrobenzene (3,4-DClNB) loading rates of 4200–7700 g m−3d−1and 6.0–70.0 g m−3d−1, andR2 had the better shock resistance and buffering capacity for the anaerobic acidification. The dechlorination for the intermediate products ofp-chloroanaline (p-ClAn) to analine (AN) occurred inR2 reactor after 45 days, whereas it did not occur inR1 after a long-term operation. The novel ZVI-based anaerobic granular sludge (ZVI–AGS) was successfully developed in the combined system, and higher microbial activities including ClNB transformation and H2/CH4production were achieved simultaneously. The dominant bacteria were closely related to the groups ofMegasphaera,Chloroflexi, andClostridium, and the majority of archaea were correlated with the groups ofMethanosarcinalesarchaeon,Methanosaetaconcilii, andMethanothrixsoehngenii, which are capable of reductively dechlorinating PCB, HCB, and TCE in anaerobic niche and EPS secretion.