Effect of chitinolytic biocontrol bacterial inoculation on soil microbiological activities and Fusarium population in rhizophere of pigeon pea (Cajanus cajan).
Effect of chitinolytic biocontrol bacterial inoculation on soil microbiological activities and Fusarium population in rhizophere of pigeon pea (Cajanus cajan).
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DOI：
10.5958/0974-0163.2018.00022.8
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Paul, S.
中科院分区：
文献类型：
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作者：
Ajinath Dukare, Ajinath Dukare;Sangeeta Paul, Sangeeta Paul;Paul, S.
采用盆栽试验研究了几丁质降解菌假单胞菌对土壤中几丁质的降解效果。菌株NS-1和芽孢杆菌属（Bacillus spp.）菌株NS-22对木豆根际土壤微生物活性的影响。测定了土壤微生物活性，包括荧光素二乙酸酯水解（FDA）、脱氢酶、磷酸酶和微生物量碳（MBC）以及镰刀菌种群数量。在假单胞菌属中发现最高的FDA水解（3.89 g荧光素释放g-1土壤hr-1）、土壤磷酸酶（0.177 g pNP释放g-1土壤hr-1）和脱氢酶活性（8.94 g TPF形成g-1土壤hr-1）。菌株NS-1处理的处理（T4）中，而在T3处理（仅Fusarium udum）中观察到最低的FDA水解和脱氢酶活性。土壤微生物量碳值在36.28-74.34 g/kg ~（-1）之间，以芽孢杆菌最高。菌株NS-22+Fusarium udum处理。在选择性镰刀菌琼脂（SFA）培养基上估计镰刀菌的种群计数，发现其范围为1.2至5.7*104 cfu g-1土壤。F的急剧减少。在T4盆栽处理中，在30 DAS观察到udum种群，其中种子在用假单胞菌属细菌化后播种。菌株NS-1，然后处理T5。这些根际细菌（假单胞菌属）。菌株NS-1和芽孢杆菌属菌株NS-22）具有良好的土壤微生物活性增强剂作用，同时对植物生长具有一定的促进作用。
A pot study was carried out to assess the effect of chitin degrading bacteria, Pseudomonas spp. strain NS-1 and Bacillus spp. strain NS-22, on soil microbiological activities in the rhizosphere of pathogen (Fusarium udum) challenged pigeon pea crop. Soil microbial activities in terms of fluorescein diacetate hydrolysis (FDA), dehydrogenase, soil phosphatase and microbial biomass carbon (MBC) and population count of Fusarium pathogen was measured. Highest FDA hydrolysis (3.89 g fluorescein released g-1 soil hr-1), soil phosphatase (0.177 g pNP released g-1 soil hr-1), and dehydrogenase activity (8.94 g TPF formed g-1 soil hr-1) were found in Pseudomonas spp. strain NS-1 treated treatment (T4), while lowest FDA hydrolysis and dehydrogenase activity were observed in T3 treatment (Fusarium udum only). Soil microbial biomass carbon value ranged from 36.28-74.34 g/kg-1 soil and was found maximum in Bacillus spp. strain NS-22+Fusarium udum treatment. Population count of Fusarium was estimated on selective Fusarium agar (SFA) media and it was found in the range of 1.2 to 5.7*104 cfu g-1 soil. Drastic reduction in F. udum population was observed at 30 DAS in T4 pot treatment where seeds were sown after bacterization with Pseudomonas spp. strain NS-1 followed by treatment T5. These rhizospheric bacteria (Pseudomonas spp. strain NS-1 & Bacillus spp. strain NS-22) revealed their potential as enhancer of soil microbial activities besides possessing biocontrol potential against Fusarium udum pathogen and plant growth promotion activities.