Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining.
Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining.
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通过 E-cadherin/CD68/CD45 三重染色检测人类肿瘤样品中的细胞内结构
DOI：
10.18632/oncotarget.4275
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发表时间：
2015-08-21
期刊：
影响因子：
--
通讯作者：
Sun Q
中科院分区：
文献类型：
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作者：
Huang H;Chen A;Wang T;Wang M;Ning X;He M;Hu Y;Yuan L;Li S;Wang Q;Liu H;Chen Z;Ren J;Sun Q
尽管细胞中细胞结构（Cell-in-cell structures，CIC）在人类肿瘤中的研究已有几十年的历史，但由于传统的H&E染色方法分辨率低，导致CIC的形成类型尚不清楚。本研究采用免疫荧光法同时用抗上皮细胞E-cadherin抗体、抗巨噬细胞CD 68抗体和抗白细胞CD 45抗体对一组人肿瘤标本进行染色，根据所检测到的细胞，我们称之为“EML法”。详细的分析显示了四种类型的CIC，肿瘤细胞或巨噬细胞分别吞噬肿瘤细胞或白细胞。有趣的是，肿瘤细胞似乎比巨噬细胞占优势（93%对7%），因为在所有CIC中检测到吞噬细胞，而内化的肿瘤细胞的总量与内化的CD 45+白细胞相当（57%对43%）。CIC谱因肿瘤而异，这可能表明不同的恶性阶段和/或炎症状况。鉴于不同类型的CIC可能对肿瘤生长产生潜在影响，因此我们建议在未来的研究中对肿瘤样本进行EML分析，以阐明CIC亚型与临床预后的相关性。
Although Cell-in-cell structures (CICs) had been documented in human tumors for decades, it is unclear what types of CICs were formed largely due to low resolution of traditional way such as H&E staining. In this work, we employed immunofluorescent method to stain a panel of human tumor samples simultaneously with antibodies against E-cadherin for Epithelium, CD68 for Macrophage and CD45 for Leukocytes, which we termed as “EML method” based on the cells detected. Detail analysis revealed four types of CICs, with tumor cells or macrophage engulfing tumor cells or leukocytes respectively. Interestingly, tumor cells seem to be dominant over macrophage (93% vs 7%) as the engulfer cells in all CICs detected, whereas the overall amount of internalized tumor cells is comparable to that of internalized CD45+ leukocytes (57% vs 43%). The CICs profiles vary from tumor to tumor, which may indicate different malignant stages and/or inflammatory conditions. Given the potential impacts different types of CICs might have on tumor growth, we therefore recommend EML analysis of tumor samples to clarify the correlation of CICs subtypes with clinical prognosis in future researches.