Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture
Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture
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DOI：
10.1128/aem.66.12.5503-5505.2000
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发表时间：
2000-12-01
影响因子：
4.4
通讯作者：
Edwards, EA
中科院分区：
文献类型：
--
作者：
Beller, HR;Edwards, EA
在厌氧条件下，甲苯矿化、产甲烷的高度浓缩的渗透细胞可以催化甲苯与富马酸的加成反应生成丁二酸苄酯。丁二酸苄酯的体外生成率是体内甲苯消耗率的85%，这是首次报道产甲烷培养物中丁二酸苄酯合成酶的活性；此前有报道称，这种活性存在于反硝化、硫酸盐还原和产氧光营养细菌中。
Permeabilized cells of a highly enriched, toluene-mineralizing, methanogenic culture catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. The specific in vitro rate of benzylsuccinate formation was >85% of the specific in vivo rate of toluene consumption, This is the first report of benzylsuccinate synthase activity in a methanogenic culture; the activity has previously been reported to occur in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria.