Makoto Shimizu, Synergistic effect of Tumor Necrosis Factor-αand hydrogen peroxide on the induction of IL-8 production in human intestinal Caco-2 cells
Makoto Shimizu, Synergistic effect of Tumor Necrosis Factor-αand hydrogen peroxide on the induction of IL-8 production in human intestinal Caco-2 cells
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Makoto Shimizu，肿瘤坏死因子-α 和过氧化氢对诱导人肠道 Caco-2 细胞产生 IL-8 的协同作用
DOI：
10.1007/s10753-010-9251-y
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发表时间：
2011
期刊：
影响因子：
5.1
通讯作者：
Mamoru Totsuka
中科院分区：
文献类型：
--
作者：
HeeSun Shin;Zhaohui Zhao;Hideo Satsu;Mamoru Totsuka
氧化应激和炎性细胞因子如TNF-α被认为参与粘膜炎症。在肠内炎症过程中，肠上皮细胞可能同时受到氧化应激和炎性细胞因子的刺激。然而，肠道炎症的实验模型仍然采用单一刺激，无论是通过氧化应激或炎症细胞因子。因此，我们使用人肠上皮Caco-2细胞进行了体外炎症研究，其中细胞被过氧化氢和TNF-α刺激，并测量了IL-8的产生作为炎症指标。TNF-α和H2 O2联合刺激的IL-8分泌、mRNA表达和转录活性均显著高于单独刺激。TNF-α和H2 O2对NF-κB信号通路（转录活性和p65核转位）的协同作用。氧化应激和TNF-α可能通过NF-κB协同促进Caco-2细胞IL-8的产生。
Oxidative stress and inflammatory cytokines such as TNF-α are thought to be involved in mucosal inflammation. The intestinal epithelium may be concurrently stimulated by both oxidative stress and inflammatory cytokines during the inflammation process in the intestines. However, experimental models for intestinal inflammation still employ single stimulation, either by an oxidative stress or inflammatory cytokine. We therefore examined anin vitroinflammation study using human intestinal epithelial Caco-2 cells, in which the cells were stimulated both by hydrogen peroxide and TNF-α, and measured the IL-8 production as an index of inflammation. The IL-8 production (secretion, mRNA expression, and transcriptional activity) induced by both TNF-α and H2O2was significantly higher than that by single stimulation. The synergistic effect of TNF-α and H2O2on the NF-κB signaling pathway (transcriptional activity and p65 nuclear translocation) was also observed. Oxidative stress and TNF-α may cooperatively enhance IL-8 productionviaNF-κB in Caco-2 cells.