STUDIES ON MANIFESTATIONS OF CANINE-DISTEMPER VIRUS-INFECTION IN AN URBAN DOG-POPULATION
STUDIES ON MANIFESTATIONS OF CANINE-DISTEMPER VIRUS-INFECTION IN AN URBAN DOG-POPULATION
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DOI：
10.1016/0378-1135(93)90190-i
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发表时间：
1993-10-01
影响因子：
3.3
通讯作者：
HENRIKSEN, P
中科院分区：
文献类型：
--
作者：
BLIXENKRONEMOLLER, M;SVANSSON, V;HENRIKSEN, P
1991年初，在哥本哈根地区的城市犬类种群中发现了犬瘟热的激增。这次暴发的特点是在部分免疫的人群中引入了致命的麻疹病毒，该病主要在年轻个体中表现出来。使用IgM ELISA对单个血清样本进行犬瘟热病毒(CDV)IgM抗体检测，证实了目前和最近在城市犬群中存在CDV感染，那里广泛使用减毒CDV疫苗。用间接免疫荧光法对66例临床疑似犬瘟患者血清中的49例进行了CDV-IgM抗体检测，并对65例结膜细胞中的27例用间接免疫荧光法进行了病毒抗原的检测。用51株抗猪瘟病毒及其相关毒株的单抗对急性和亚急性临床分离株的抗原组成进行了研究。这些分离株表现出均一的反应模式，并具有CDV原型的全部抗原性。大多数病例是在未接种疫苗的狗只和接种记录不明或不明的个人中诊断出来的。然而，在接种疫苗的个人中也诊断出严重的临床病例。
An upsurge of canine distemper was recognized at the beginning of 1991 in the urban dog population of the Copenhagen area. The outbreak had the characteristics of a virulent morbillivirus introduction in a partly immune population, where the disease primarily was manifested in young individuals.Testing of single serum samples for the presence of canine distemper Virus (CDV) IgM antibodies using an IgM ELISA confirmed current and recent CDV infections in an urban dog population, where the use of attenuated CDV vaccines was widespread. In 49 out of 66 sera from clinical cases suspected of canine distemper we detected CDV IgM antibodies, as compared to the detection of viral antigen by indirect immunofluorescence in 27 of 65 specimens of conjunctival cells. The antigenic make-up of isolates from acute and subacute clinical cases was investigated with a panel of 51 monoclonal antibodies directed against CDV and the related phocine distemper virus. The isolates exhibited an homogenous reaction pattern and shared overall antigenic characteristics of the CDV prototype. The majority of cases were diagnosed among unvaccinated dogs and individuals with unknown or obscure vaccination record. However, severe clinical cases were also diagnosed in vaccinated individuals.