Melatonin Mediates Monochromatic Light-induced Insulin-like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane Receptors
Melatonin Mediates Monochromatic Light-induced Insulin-like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane Receptors
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褪黑素介导单色光诱导鸡肝脏胰岛素样生长因子 1 分泌：膜受体的参与
DOI：
10.1111/php.12594
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发表时间：
2016-07-01
影响因子：
3.3
通讯作者：
Chen, Yaoxing
中科院分区：
文献类型：
--
作者：
Li, Suqi;Cao, Jing;Chen, Yaoxing
单色光通过促进胰岛素样生长因子1（IGF-1）的分泌影响肉鸡骨骼肌卫星细胞的增殖和分化。然而，褪黑激素（MEL）介导的单色光是否影响IGF-1的分泌仍不清楚。将刚出壳的肉仔鸡分为正常组、假手术组和松果体切除组，分别暴露于发光二极管系统发出的蓝光（BL）、绿色（GL）、红光（RL）和白色光（WL）中14 d。结果表明，与BL和RL相比，GL能有效促进肝脏MEL和IGF-1的分泌，增殖细胞核抗原和MEL受体亚型Mel 1a、Mel 1b和Mel 1c的表达。MEL与IGF-1呈正相关（r = 0.834）。然而，松果体切除术后，这些参数下降，GL和其他单色光治疗之间没有差异。在体外，外源性MEL增加肝细胞增殖和IGF-1分泌。同时，选择性Mel 1c拮抗剂哌唑嗪抑制MEL增强，非选择性Mel 1a/Mel 1b拮抗剂Luzindole次之，选择性Mel 1b拮抗剂4-phenyl-2-propionamideotetralin不抑制MEL增强。这些发现表明MEL通过Mel 1c介导单色光诱导鸡肝脏分泌IGF-1，而Mel 1a可能参与了这一过程。
Monochromatic lights influenced the proliferation and differentiation of skeletal satellite cells in broilers by the enhancement of insulin-like growth factor 1 (IGF-1) secretion. However, whether melatonin (MEL)-mediated monochromatic lights influenced the IGF-1 secretion remains unclear. Newly hatched broilers, including intact, sham operation and pinealectomy groups, were exposed to blue (BL), green (GL), red (RL) and white light (WL) from a light-emitting diode system for 14 days. The results showed that GL effectively promoted the secretion of MEL and IGF-1, the expression of proliferating cell nuclear antigen and MEL receptor subtypes Mel1a, Mel1b and Mel1c in the liver compared to BL and RL in vivo. Moreover, those was a positive correlation between MEL and IGF-1 (r = 0.834). After pinealectomy, however, these parameters declined, and there were no differences between GL and other monochromatic light treatments. In vitro, exogenous MEL increased hepatocyte proliferation and IGF-1 secretion. Meanwhile, the MEL enhancements were suppressed by prazosin (selective Mel1c antagonist), followed by luzindole (nonselective Mel1a/Mel1b antagonist), but not suppressed by 4-phenyl-2-propionamideotetralin (selective Mel1b antagonist). These findings demonstrated that MEL mediated the monochromatic light-induced secretion of IGF-1 in chicks' livers by Mel1c and that Mel1a may be involved in this process.