High resolution micro arthrography of hard and soft tissues in a murine model
High resolution micro arthrography of hard and soft tissues in a murine model
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DOI：
10.1016/j.joca.2012.05.004
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发表时间：
2012-09-01
影响因子：
7
通讯作者：
Cardoso, L.
中科院分区：
文献类型：
--
作者：
Gu, X. I.;Palacio-Mancheno, P. E.;Cardoso, L.
目的：高分辨率微型计算机断层扫描（mu CT）的最新发展使小动物关节软组织成像成为可能。然而，由于软组织和滑液的质量密度相似，mu CT图像无法区分软组织和滑液，限制了软组织体积和厚度的3D评估。本研究旨在评价一种铬酸铅造影剂在大鼠膝关节离体CT关节造影中的应用价值。设计：将完整的大鼠胫股关节注射不同浓度的造影剂，使用2.7 μ m各向同性体素大小的CT成像。使用自动化程序测量软骨厚度，根据组织学测量结果进行验证，并作为mu CT图像分辨率的函数进行分析。我们还分析了内侧半月板失稳（DMM）术后4周胫股关节的硬软组织变化。结果：造影剂在整个膝关节内扩散良好，不穿透组织，因此在软组织和滑液间隙之间提供了高对比度。软骨厚度分析显示组织学和关节造影入路高度相似（R-2 = 0.90）。术后4周，骨赘（Oph）和软骨溃疡在mu CT上可见，骨小梁疏松度轻微增加，骨小梁厚度减少。结论：基于铅色层析剂的造影剂可以区分完整膝关节的滑液和软组织，从而可以使用高分辨率mu CT对完整和DMM胫股关节的硬软组织进行定性和定量评估。(c) 2012年国际骨关节炎研究学会。Elsevier Ltd.出版。版权所有。
Objective: Recent developments on high resolution micro computed tomography (mu CT) allow imaging of soft tissues in small animal joints. Nevertheless, mu CT images cannot distinguish soft tissues from synovial fluid due to their similar mass density, limiting the 3D assessment of soft tissues volume and thickness. This study aimed to evaluate a lead chromate contrast agent for mu CT arthrography of rat knee joints ex vivo.Design: Intact tibiofemoral rat joints were injected with the contrast agent at different concentrations and imaged using a mu CT at 2.7 mu m isotropic voxel size. Cartilage thickness was measured using an automated procedure, validated against histological measurements, and analyzed as a function of mu CT image resolution. Changes in hard and soft tissues were also analyzed in tibiofemoral joints 4 weeks after surgical destabilization of the medial meniscus (DMM).Results: The contrast agent diffused well throughout the whole knee cavity without penetrating the tissues, therefore providing high contrast at the boundaries between soft tissues and synovial fluid space. Thickness analysis of cartilage demonstrated a high similarity between histology and mu-arthrography approaches (R-2 = 0.90). Four weeks after surgical DMM, the development of osteophytes (Oph) and cartilage ulcerations was recognizable with mu CT, as well as a slight increase in trabecular bone porosity, and decrease in trabecular thickness.Conclusions: A lead chromate-based contrast agent allowed discriminating the synovial fluid from soft tissues of intact knee joints, and thus made possible both qualitative and quantitative assessment of hard and soft tissues in both intact and DMM tibiofemoral joints using high resolution mu CT. (c) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.