Quantitative analysis of colistin A and colistin B in plasma and culture medium using a simple precipitation step followed by LC/MS/MS
Quantitative analysis of colistin A and colistin B in plasma and culture medium using a simple precipitation step followed by LC/MS/MS
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DOI：
10.1016/j.jpba.2008.12.016
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发表时间：
2009-04-05
影响因子：
3.4
通讯作者：
Friberg, Lena E.
中科院分区：
文献类型：
--
作者：
Jansson, Britt;Karvanen, Matti;Friberg, Lena E.
建立了测定血浆和培养上清液中粘菌素A和粘菌素B的分析方法。蛋白质用含0.1%三氟乙酸(TFA)的乙腈(ACN)沉淀后，上清液用0.03%TFA稀释。采用超声波phere C18色谱柱，4.6 mm×250 mm，5µm粒径，流动相为25%ACN-0.03%TFA，串联质谱仪检测。该仪器工作在电喷雾负离子模式，前体-产物离子对粘菌素A为m/z 1167.7-1079.6，粘菌素B为m/z 1153.7-gt；1065.6。百亩L血浆中粘菌素A和粘菌素B的定量下限分别为19.4和10.5 ng/mL，变异系数(CV)为
An analytical method for quantitation of colistin A and colistin B in plasma and culture medium is described. After protein precipitation with acetonitrile (ACN) containing 0.1% trifluoroacetic acid (TFA), the supernatants were diluted with 0.03% TFA. The compounds were separated on an Ultrasphere C18 column, 4.6 mm x 250 mm, 5 mu m particle size with a mobile phase consisting of 25% ACN in 0.03% TFA and detected with tandem mass spectrometry. The instrument was operating in ESI negative ion mode and the precursor-product ion pairs were m/z 1167.7 -> 1079.6 for colistin A and m/z 1153.7 -> 1065.6 for colistin B. The lower limit of quantification (LLOQ) for 100 mu L plasma was 19.4 and 10.5 ng/mL for colistin A and B, respectively, with CV