Measurement of single channel currents from cardiac gap junctions.
Measurement of single channel currents from cardiac gap junctions.
复制标题
测量心脏间隙连接处的单通道电流。
DOI：
10.1126/science.2426781
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
DeHaan,RL
中科院分区：
文献类型：
--
作者：
Veenstra,RD;DeHaan,RL
心脏间隙连接由在细胞与细胞接触点穿过细胞间裂隙连接的完整膜蛋白阵列组成。这些连接蛋白被认为形成孔，离子可以通过这些孔从胞质溶胶扩散到胞质溶胶。用两个独立的膜片钳电路监测成对胚胎心脏细胞的全细胞电流，研究了单个缝隙连接通道的特性。这些通道的电导率约为165皮西门子，并经历独立于电压的自发打开和关闭。通道活性和宏观连接电导均被解偶联剂1-辛醇降低。
Cardiac gap junctions consist of arrays of integral membrane proteins joined across the intercellular cleft at points of cell-to-cell contact. These junctional proteins are thought to form pores through which ions can diffuse from cytosol to cytosol. By monitoring whole-cell currents in pairs of embryonic heart cells with two independent patch-clamp circuits, the properties of single gap junction channels have been investigated. These channels had a conductance of about 165 picosiemens and underwent spontaneous openings and closings that were independent of voltage. Channel activity and macroscopic junctional conductance were both decreased by the uncoupling agent 1-octanol.