OAR, A 115-KILODALTON MEMBRANE-PROTEIN REQUIRED FOR DEVELOPMENT OF MYXOCOCCUS-XANTHUS
OAR, A 115-KILODALTON MEMBRANE-PROTEIN REQUIRED FOR DEVELOPMENT OF MYXOCOCCUS-XANTHUS
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DOI：
10.1128/jb.175.15.4756-4763.1993
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发表时间：
1993-08-01
影响因子：
3.2
通讯作者：
INOUYE, S
中科院分区：
文献类型：
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作者：
MARTINEZCANAMERO, M;MUNOZDORADO, J;INOUYE, S
黄色粘球菌（Myxococcus xanthus）是一种革兰氏阴性细菌，在营养饥饿时形成多细胞子实体。发现该细菌含有通过蔗糖密度梯度离心与内膜部分分离的115-kDa膜蛋白。克隆了该蛋白的基因，并测定了其DNA序列。推导的氨基酸序列由1，061个残基组成。这种蛋白质含有一个推定的信号序列和许多短片段，发现分散在整个蛋白质中，与大肠杆菌的主要外膜蛋白OmpA具有序列相似性。因此，该基因被命名为oar（OmpA相关蛋白）。在oar终止密码子下游36个碱基处发现了第二个开放阅读框。该开放阅读框编码236个残基的蛋白质，并含有推定的脂蛋白信号序列。桨状中断突变（DELTAoar）对营养生长没有影响，但在发育过程中引起异常形态发生，并减少粘孢子形成。当用光学显微镜检查时，DELTAoar细胞不能在发育琼脂上聚集，表明Oar是发育过程中细胞聚集所必需的。
Myxococcus xanthus is a developmental gram-negative bacterium which forms multicellular fruiting bodies upon nutrient starvation. This bacterium was found to contain a 115-kDa membrane protein which separated with the inner membrane fraction by sucrose density gradient centrifugation. The gene for this protein was cloned, and its DNA sequence was determined. The deduced amino acid sequence consists of 1,061 residues. This protein contains a putative signal sequence and many short segments, found scattered throughout the entire protein, that have sequence similarities with OmpA, a major outer membrane protein of Escherichia coli. Thus, the gene was designated oar (OmpA-related protein). A second open reading frame was found 36 bases downstream of the oar termination codon. This open reading frame encodes a protein of 236 residues and contains a putative lipoprotein signal sequence. An oar disruption mutation (DELTAoar) showed no effect on vegetative growth but caused abnormal morphogenesis during development and reduced myxospore formation. When examined with a light microscope, DELTAoar cells were unable to aggregate on developmental agar, indicating that Oar is required for cellular adhesiveness during development.