Purification and Storage of Ribulose 1,5-bisphosphate Carboxylase from Rice Leaves
Purification and Storage of Ribulose 1,5-bisphosphate Carboxylase from Rice Leaves
复制标题
稻叶核酮糖 1,5-二磷酸羧化酶的纯化和储存
DOI：
10.1093/oxfordjournals.pcp.a076623
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发表时间：
1983
影响因子：
4.9
通讯作者：
K. Ohira
中科院分区：
文献类型：
--
作者：
A. Makino;T. Mae;K. Ohira
从水稻叶片中分离纯化了核酮糖1，5-二磷酸（RuBP）羧化酶。通过在整个纯化过程中使用含有12.5%（v/v）甘油的缓冲液，保护酶免受冷不稳定性，并以高产率（5.5mg/g鲜重）获得。通过温度预处理/储存，纯化的酶表现出不同的CO2/Mg 2 +-活化速率。纯化的酶在4°C下含有12.5%（v/v）甘油的磷酸盐缓冲液中或在−20°C下含有50%（v/v）甘油的磷酸盐缓冲液中稳定至少一年。
Ribulose 1,5-bisphosphate (RuBP) carboxylase was purified from rice leaves. By using a buffer containing 12.5% (v/v) glycerol throughout purification, the enzyme was protected from cold lability and was obtained at a high yield (5.5 mg/g fresh wt). The purified enzyme exhibited different rates of CO2/Mg2+-activation by temperature pretreatment/storage.The purified enzyme was stable for at least one year in phosphate buffer containing 12.5% (v/v) glycerol at 4°C or 50% (v/v) glycerol at −20°C.