Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys.
Direct physical contact between intercalated cells in the distal convoluted tubule and the afferent arteriole in mouse kidneys.
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小鼠肾脏远端曲管中的闰细胞与传入小动脉之间的直接物理接触
DOI：
10.1371/journal.pone.0070898
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhai XY
中科院分区：
文献类型：
--
作者：
Ren H;Liu NY;Andreasen A;Thomsen JS;Cao L;Christensen EI;Zhai XY
最近在肾脏的生理学研究提出了存在一个二级反馈机制，称为“串扰”定位后的致密斑。这一新发现的肾单位小管和其自身的传入小动脉之间的串扰接触可能会彻底改变我们对肾血管阻力和电解质调节的理解。然而，由于缺乏直接和全面的形态学证据，这种串扰机制的性质仍有争议。它在肾单位上的确切位置、在不同类型肾单位中的普遍性以及所涉及的细胞类型尚不清楚。为了解决这些问题，计算机辅助三维肾单位示踪与直接免疫组织化学塑料切片和电子显微镜相结合。皮质中的“随机”接触通过追踪识别并排除。我们共研究了168个来自所有皮质区域的肾单位。结果表明，串扰接触是存在的，它只存在于某些肾单位（90%的短环和75%的长环肾单位）。串扰接触总是发生在特定的位置-远曲小管的最后10%。重要的是，我们第一次证明了在接触部位的小管壁中发现的细胞总是非A-nonB型的嵌入细胞。总之，目前的工作证实了后致密斑物理串扰接触的存在。
Recent physiological studies in the kidney proposed the existence of a secondary feedback mechanism termed ‘crosstalk’ localized after the macula densa. This newly discovered crosstalk contact between the nephron tubule and its own afferent arteriole may potentially revolutionize our understanding of renal vascular resistance and electrolyte regulation. However, the nature of such a crosstalk mechanism is still debated due to a lack of direct and comprehensive morphological evidence. Its exact location along the nephron, its prevalence among the different types of nephrons, and the type of cells involved are yet unknown. To address these issues, computer assisted 3-dimensional nephron tracing was applied in combination with direct immunohistochemistry on plastic sections and electron microscopy. ‘Random’ contacts in the cortex were identified by the tracing and excluded. We investigated a total of 168 nephrons from all cortical regions. The results demonstrated that the crosstalk contact existed, and that it was only present in certain nephrons (90% of the short-looped and 75% of the long-looped nephrons). The crosstalk contacts always occurred at a specific position – the last 10% of the distal convoluted tubule. Importantly, we demonstrated, for the first time, that the cells found in the tubule wall at the contact site were always type nonA-nonB intercalated cells. In conclusion, the present work confirmed the existence of a post macula densa physical crosstalk contact.