Dual Phenotype Kisspeptin-Dopamine Neurones of the Rostral Periventricular Area of the Third Ventricle Project to Gonadotrophin-Releasing Hormone Neurones
Dual Phenotype Kisspeptin-Dopamine Neurones of the Rostral Periventricular Area of the Third Ventricle Project to Gonadotrophin-Releasing Hormone Neurones
复制标题
DOI：
10.1111/j.1365-2826.2011.02107.x
复制
发表时间：
2011-04-01
影响因子：
3.2
通讯作者：
Herbison, A. E.
中科院分区：
文献类型：
--
作者：
Clarkson, J.;Herbison, A. E.
神经肽kisspeptin及其G蛋白偶联受体Gpr 54是生育的关键调节因子。啮齿类动物中存在两个主要的kisspeptin神经元群体：一个在第三脑室的头侧脑室周区（RP 3V），另一个在弓状核。Kisspeptin神经元的RP 3V群体对于促黄体生成激素激增的产生至关重要，促黄体生成激素激增驱动女性排卵。RP 3V kisspeptin神经元是性二态的，女性比男性有更多的神经元，它们投射到促性腺激素释放激素（GnRH）神经元。RP 3V中表达酪氨酸羟化酶（TH）的神经元也是性二态的，并被认为投射到GnRH神经元。在本研究中，我们研究了kisspeptin和TH肽的共表达的RP 3V的dioestrous和pro-oestrous雌性小鼠。我们还研究了kisspeptin和TH肽是否与GnRH神经元在吻侧视前区（rPOA）的终端并置共定位。大约一半的kisspeptin神经元中的RP 3V被发现也表达TH，反之亦然，虽然有没有小鼠之间的差异，在间情期或发情前期。大多数（95%）的GnRH神经元的rPOA表现出密切的贴壁从kisspeptin纤维，而只有四分之一表现出密切的贴壁从TH纤维。许多TH与GnRH神经元的紧密结合共表达kisspeptin（62-86%），尽管这些双标记结合占GnRH神经元上所有kisspeptin结合的< 20%。GnRH神经元与kisspeptin，TH和双标记的appositions的百分比之间没有差异dioestrous和pro-oestrous小鼠。这些结果表明，kisspeptin神经元表达多巴胺神经支配的rPOA的GnRH神经元的亚群。
The neuropeptide kisspeptin and its G-protein-coupled receptor, Gpr54, are critical regulators of fertility. Two major populations of kisspeptin neurones exist in the rodent: one in the rostral periventricular area of the third ventricle (RP3V) and another in the arcuate nucleus. The RP3V population of kisspeptin neurones is crucial for the generation of the luteinising hormone surge that drives ovulation in females. The RP3V kisspeptin neurones are sexually dimorphic, with many more neurones in females than males, and they project to gonadotrophin-releasing hormone (GnRH) neurones. Tyrosine hydroxylase (TH) expressing neurones in the RP3V are also sexually dimorphic and are assumed to project to GnRH neurones. In the present study, we examined the coexpression of kisspeptin and TH peptides in the RP3V of dioestrous and pro-oestrous female mice. We also investigated whether kisspeptin and TH peptides colocalised in terminal appositions with GnRH neurones in the rostral preoptic area (rPOA). Approximately half of the kisspeptin neurones in the RP3V were found to also express TH and vice versa, although there was no difference between mice in dioestrus or pro-oestrus. The majority (95%) of GnRH neurones in the rPOA exhibited a close apposition from a kisspeptin fibre, whereas only one quarter exhibited a close apposition from a TH fibre. Many of the TH close appositions with GnRH neurones coexpressed kisspeptin (62-86%), although these dual-labelled appositions comprised < 20% of all kisspeptin appositions on GnRH neurones. The percentage of GnRH neurones with kisspeptin, TH and double-labelled appositions did not differ between dioestrous and pro-oestrous mice. These findings indicate that a subpopulation of kisspeptin neurones expressing dopamine innervate GnRH neurones in the rPOA.