Microchannel wall coatings for protein separations by capillary and chip electrophoresis
Microchannel wall coatings for protein separations by capillary and chip electrophoresis
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DOI：
10.1002/elps.200390029
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发表时间：
2003-01-01
期刊：
影响因子：
2.9
通讯作者：
Barron, AE
中科院分区：
文献类型：
--
作者：
Doherty, EAS;Meagher, RJ;Barron, AE
微通道壁涂层在毛细管电泳和基于芯片的生物分子电泳分析中的必要性是众所周知的。调节或消除EOF和防止分析物吸附对于在微通道内快速、有效地分离蛋白质和DNA至关重要，微通道壁涂层和其他壁修饰对于蛋白质分离尤其重要，无论是在熔融二氧化硅毛细管中，还是在玻璃或聚合物微流体装置中。本文介绍了共价键合聚合物涂料、物理吸附聚合物涂料和小分子添加剂三大类微通道壁涂料的最新进展。我们还简要回顾了聚合物微流控器件的改进方法。在每一类壁面涂料中，我们讨论了那些用于消除EOF、调节EOF、防止分析物吸附或执行多种功能的壁面涂料。对这一领域最有前途的最新发展的知识应用将允许分离复杂的蛋白质混合物和开发新的微通道壁修饰。
The necessity for microchannel wall coatings in capillary and chip-based electrophoretic analysis of biomolecules is well understood. The regulation or elimination of EOF and the prevention of analyte adsorption is essential for the rapid, efficient separation of proteins and DNA within microchannels, Microchannel wall coatings and other wall modifications are especially critical for protein separations, both in fused-silica capillaries, and in glass or polymeric microfluidic devices, In this review, we present a discussion of recent advances in microchannel wall coatings of three major classes - covalently linked polymeric coatings, physically adsorbed polymeric coatings, and small molecule additives. We also briefly review modifications useful for polymeric microfluidic devices. Within each category of wall coatings, we discuss those used to eliminate EOF, to tune EOF, to prevent analyte adsorption, or to perform multiple functions. The knowledgeable application of the most promising recent developments in this area will allow for the separation of complex protein mixtures and for the development of novel microchannel wall modifications.