Preparation of Bi0.15Fe0.15TiO2 Nanocomposites for the Highly Selective Enrichment of Phosphopeptides
Preparation of Bi0.15Fe0.15TiO2 Nanocomposites for the Highly Selective Enrichment of Phosphopeptides
复制标题
用于高选择性富集磷酸肽的 Bi0.15Fe0.15TiO2 纳米复合材料的制备
DOI：
10.1021/acs.analchem.8b00606
复制
发表时间：
2018
影响因子：
7.4
通讯作者：
Cai Qingyun
中科院分区：
文献类型：
--
作者：
Zhen Deshuai;Gao Chan;Zhu Baode;Zhou Qian;Li Chenyi;Chen Ping;Cai Qingyun
采用改进的溶胶凝胶法首次合成了新型Bi0.15Fe0.15TiO2纳米复合材料(B0.15F0.15Tns)，并成功地应用于从消化后的蛋白质混合液和实际样品(人肝组织蛋白提取物)中选择性地提取和富集磷酸肽。在二氧化钛中掺入铋和铁，可以显著提高富集效率和选择性。与市售的二氧化钛萃取剂相比，B0.15F0.15Tns具有更低的检测下限(2×10-9M)和更高的选择性，且重量比为1：1200(磷酸肽/非磷肽)。此外，经B0.15F0.15Tns富集后，从人肝裂解液中鉴定出总共223个磷酸化位点。另外，B_(0.15)F_(0.15)Tns的合成非常简单，产率高，价格低廉。
Novel Bi0.15Fe0.15TiO2nanocomposites (B0.15F0.15TNs) were synthesized for the first time by a modified sol–gel technology and successfully applied to selective extraction and enrichment of phosphopeptides from digested protein mixture solutions and real samples (tissue protein extract from human liver). The codoping of Bi and Fe into TiO2results in a significant enhancement in both the enrichment efficiency and selectivity. Compared with the commercial available TiO2extractant, the proposed B0.15F0.15TNs possess a lower detection limit (2 × 10–9M) and higher selectivity at a low weight ratio of 1:1200 (phosphopeptides/nonphosphopeptides). Additionally, a total of 223 phosphorylation sites were identified from the human liver lysate after enrichment by the B0.15F0.15TNs. In addition, the synthesis of B0.15F0.15TNs is quite easy, of high yield, and inexpensive.