Testing the polar auxin transport model with a selective plasma membrane H+-ATPase inhibitor
Testing the polar auxin transport model with a selective plasma membrane H+-ATPase inhibitor
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用选择性质膜 H~ -ATPase 抑制剂测试极性生长素转运模型
DOI：
10.1111/jipb.13256
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发表时间：
2022-05-27
影响因子：
11.4
通讯作者：
Lei, Xiaoguang
中科院分区：
文献类型：
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作者：
Yang, Yongqing;Liu, Xiaohui;Lei, Xiaoguang
生长素在植物激素中是独特的，因为其功能需要在植物细胞中进行极化运输。提出了一个化学渗透模型来解释生长素极性运输是如何通过质膜H+-腺苷三磷酸酶（ATP酶）建立的H+跨膜梯度来实现的。然而，一个经典的遗传方法突变的PM H+-ATP酶成员没有导致消融极性生长素分布，可能是由于在这个基因家族的功能冗余。为了证实PM H+-ATP酶在生长素极性运输模型中的重要作用，我们采用了化学遗传学方法。通过化学筛选，我们确定了质子抑制素-1（PS-1），一种选择性的小分子PM H+-ATP酶活性抑制剂，抑制生长素运输。对转基因植株和酵母菌株的测定表明，PM H+-ATP酶的活性影响生长素的吸收以及生长素向顶和向基的极性运输。我们建议PS-1可以作为一个工具来询问PM H+-ATP酶的功能。我们的研究结果支持化学渗透模型，其中PM H+-ATP酶本身在极性生长素运输中起着重要作用。
Auxin is unique among plant hormones in that its function requires polarized transport across plant cells. A chemiosmotic model was proposed to explain how polar auxin transport is derived by the H+ gradient across the plasma membrane (PM) established by PM H+-adenosine triphosphatases (ATPases). However, a classical genetic approach by mutations in PM H+-ATPase members did not result in the ablation of polar auxin distribution, possibly due to functional redundancy in this gene family. To confirm the crucial role of PM H+-ATPases in the polar auxin transport model, we employed a chemical genetic approach. Through a chemical screen, we identified protonstatin-1 (PS-1), a selective small-molecule inhibitor of PM H+-ATPase activity that inhibits auxin transport. Assays with transgenic plants and yeast strains showed that the activity of PM H+-ATPases affects auxin uptake as well as acropetal and basipetal polar auxin transport. We propose that PS-1 can be used as a tool to interrogate the function of PM H+-ATPases. Our results support the chemiosmotic model in which PM H+-ATPase itself plays a fundamental role in polar auxin transport.