Spatial regulation of cytokinesis by the Kin1 and Pom1 kinases in fission yeast
Spatial regulation of cytokinesis by the Kin1 and Pom1 kinases in fission yeast
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DOI：
10.1007/s00294-006-0099-5
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发表时间：
2006-12-01
期刊：
影响因子：
2.5
通讯作者：
Le Goff, Xavier
中科院分区：
文献类型：
--
作者：
La Carbona, Stephanie;Le Goff, Xavier
细胞分裂需要与有丝分裂紧密的时空协调，以确保细胞分裂过程中遗传信息的正确分离。在裂殖酵母中，肌动球蛋白收缩环在有丝分裂中组装并决定胞质分裂的位点。在这里，我们研究了 Kin1 和 Pom1（两种保守的裂殖酵母激酶）在细胞分裂中的功能。我们发现 kin1 Delta 与 pom1 Delta 具有综合致死性，因为双突变细胞在有丝分裂期间无法在空间上组织肌动球蛋白环，导致隔膜合成异常以及有丝分裂后核在同一细胞室中积累。有丝分裂时细胞中心 Rlc1-GFP 环的组装也受到损害。在 tea1 Delta kin1 Delta 突变体中也观察到类似的细胞因子缺陷。此外，在 pom1 Delta 菌株中观察到异常分隔和核积累，其中 Kin1 水平下调或上调。因此，严格控制 Kin1 水平对于确保 pom1 Delta 背景中细胞的准确分裂至关重要。由于激酶不能相互替代，Kin1 和 Pom1 具有不同的互补功能。我们发现 Kin1 是间期和有丝分裂完成后 F-肌动蛋白极化所必需的，并且该功能可能对于 pom1 Delta 背景中的胞质分裂至关重要。
Cytokinesis requires a tight spatio-temporal coordination with mitosis to ensure proper segregation of the genetic information during cell division. In fission yeast, an actomyosin contractile ring is assembled in mitosis and dictates the site of cytokinesis. Here we investigated the functions of Kin1 and Pom1, two conserved fission yeast kinases, in cell division. We found that kin1 Delta is synthetically lethal with pom1 Delta because double mutant cells fail to spatially organize the actomyosin ring during mitosis, leading to aberrant septum synthesis and accumulation of post-mitotic nuclei in the same cell compartment. Assembly of an Rlc1-GFP ring in the cell center at mitosis is also compromised. Similar cytokinetic defects are observed in a tea1 Delta kin1 Delta mutant. Furthermore, aberrant septation and nuclear accumulation are observed in a pom1 Delta strain in which the Kin1 level is either down or up-regulated. Thus, a tight control of Kin1 level is critical for ensuring accurate cell division in a pom1 Delta background. Since none of the kinases can substitute for each other, Kin1 and Pom1 have distinct complementary functions. We show that Kin1 is required for F-actin polarization in interphase and after completion of mitosis and this function may be essential for cytokinesis in a pom1 Delta background.