Optimization of nutrients for gellan gum production by Sphingomonas paucimobilis ATCC-31461 in molasses based medium using response surface methodology
Optimization of nutrients for gellan gum production by Sphingomonas paucimobilis ATCC-31461 in molasses based medium using response surface methodology
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DOI：
10.1016/j.biortech.2006.03.012
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发表时间：
2007-03-01
影响因子：
11.4
通讯作者：
Upadhyay, S. N.
中科院分区：
文献类型：
--
作者：
Banik, R. M.;Santhiagu, A.;Upadhyay, S. N.
研究了少动鞘氨醇单胞菌ATCC-31461生产结冷胶的糖蜜培养基。采用Placket-Burman设计准则研究了不同营养添加剂对糖蜜生产结冷胶的影响。在所测试的20个变量中，糖蜜、胰蛋白胨、酪蛋白氨基酸、正磷酸氢二钠和氯化锰对结冷胶产量有显著影响。中心复合设计用于确定Placket-Burman设计中获得的显著变量的最佳浓度。最适合生产结冷胶的培养基组成为（g/l）：糖蜜-112.5;胰蛋白胨-1;酪蛋白氨基酸-1;二钠。正磷酸氢-1;氯化锰-0.947;最佳结冷胶产量为13.814 g/L。(c)2006爱思唯尔有限公司保留所有权利。
A molasses based medium for the production of gellan by Sphingomonas paucimobilis ATCC-31461 was developed. Placket-Burman design criterion was applied to study the effect of various nutrient supplements on gellan production using molasses. Among the 20 variables tested, molasses, tryptone, casaminoacid, disodium hydrogen orthophosphate and manganese chloride showed significant effect on gellan production. A central composite design was applied to determine the optimum concentrations of the significant variables obtained from Placket-Burman design. Most suitable medium composition for production of gellan was (g/l): molasses-112.5; tryptone-1; casaminoacid-1; disodium. hydrogen orthophosphate-1; manganese chloride-0.947 and the optimum gellan production was 13.814 g/l. (c) 2006 Elsevier Ltd. All rights reserved.