SKELETAL DEVELOPMENT AND FORMATION OF OSTEOCLAST-LIKE CELLS FROM INSITU PROGENITORS IN FETAL MOUSE METATARSALS CULTURED IN CHEMICALLY DEFINED MEDIUM
SKELETAL DEVELOPMENT AND FORMATION OF OSTEOCLAST-LIKE CELLS FROM INSITU PROGENITORS IN FETAL MOUSE METATARSALS CULTURED IN CHEMICALLY DEFINED MEDIUM
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DOI：
10.1016/0169-6009(91)90028-x
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发表时间：
1991-03-01
期刊：
影响因子：
--
通讯作者：
MARKS, SC
中科院分区：
文献类型：
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作者：
MINKIN, C;STJAMES, S;MARKS, SC
描述了一种体外模型系统，使用跖骨外植体从15天的小鼠胚胎（E15）无血清化学成分确定的培养基中培养，研究胎儿骨骼发育，特别强调从头破骨细胞的形成。 在体外观察到的正常模式的生长和分化，通过超微结构和形态测量学评估，表现出一个允许的局部环境，复制生理的时间和空间的关系，存在于体内。 E15跖骨中存在的定向破骨细胞祖细胞群体形成具有细胞化学和超微结构特征以及形成动力学的破骨细胞和前体细胞，其与体内发生的相似。 破骨细胞形成对添加的1，25（OH）2D3的作用的响应性说明，受控操作使人们能够利用该系统来研究细胞因子、生长因子和促骨激素在骨骼发育和破骨细胞个体发育中的作用。
An in vitro model system is described, using metatarsal explants from 15-day mouse embryos (E15) cultured in serumless chemically defined medium, to study fetal skeletal development with particular emphasis on de novo osteoclast formation. The normal pattern of growth and differentiation observed in vitro, assessed by ultrastructure and morphometry, demonstrate a permissive local environment which replicates physiologic temporal and spatial relationships which exist in vivo. The population of committed osteoclast progenitors present in E15 metatarsals form osteoclasts and precursors which have cytochemical and ultrastructural features, as well as kinetics of formation, that are similar to that which occurs in vivo. The responsiveness of osteoclast formation to the effects of added 1,25(OH)2D3 illustrates that controlled manipulation enables one to exploit the system for investigating the role of cytokines, growth factors and osteotropic hormones in skeletal development and osteoclast ontogeny.