A Study on Effect of Electroacupuncture on Gene Expression in Hypothalamus of Rats with Stress-Induced Prehypertension Based on Gene Chip Technology.
A Study on Effect of Electroacupuncture on Gene Expression in Hypothalamus of Rats with Stress-Induced Prehypertension Based on Gene Chip Technology.
复制标题
基于基因芯片技术的电针对应激性高血压前期大鼠下丘脑基因表达影响的研究
DOI：
10.1155/2015/621237
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Guo C
中科院分区：
文献类型：
--
作者：
Xie X;Guo Y;Liu Q;Wang Z;Guo C
客观的。探讨电针（EA）对应激性高血压前期大鼠下丘脑基因表达的影响，并试图利用基因芯片技术揭示其生物学机制。方法。采用足部电击与产生的噪声相结合的方法制备应激性高血压大鼠模型。成型周期持续14天，模型制备过程中对模型+电针组进行电针干预。采用Rat Gene 2.0 Array技术测定基因表达谱，并采用实时荧光定量PCR方法对筛选出的关键基因进行验证。结果。与空白组相比，模型组有234个基因上调，73个基因下调。与模型组相比，模型+电针组有110个基因上调，273个基因下调。 HSPB1、P2RX4、PPP1R14A、TH等关键基因PCR结果与基因芯片检测结果一致。结论。电针可显着降低应激性高血压前期大鼠的血压并影响其下丘脑基因表达谱。与血管平滑肌收缩、Ca2+浓度、交感神经兴奋性相关的基因及其信号转导通路可能参与了EA的降压机制。
Objective. To explore the effect of electroacupuncture (EA) on gene expression in the hypothalamus of rats with stress-induced prehypertension and try to reveal its biological mechanism with gene chip technology. Methods. The stress-induced hypertensive rat model was prepared by combining electric foot-shocks with generated noise. Molding cycle lasted for 14 days and EA intervention was applied on model + EA group during model preparation. Rat Gene 2.0 Array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time fluorescence quantitative PCR method. Results. Compared with the blank group, 234 genes were upregulated and 73 were downregulated in the model group. Compared with the model group, 110 genes were upregulated and 273 genes were downregulated in model + EA group. The PCR results of the key genes including HSPB1, P2RX4, PPP1R14A, and TH are consistent with that of gene chip test. Conclusion. EA could significantly lower blood pressure of stress-induced prehypertension rats and affect its gene expression profile in hypothalamus. Genes and their signal transduction pathway that related to the contraction of vascular smooth muscle, concentration of Ca2+, and excitability of sympathetic nerve may be involved in EA's antihypertensive mechanism.