Evidence that the same structural gene encodes testicular and adrenal 3 beta-hydroxysteroid dehydrogenase-isomerase.
Evidence that the same structural gene encodes testicular and adrenal 3 beta-hydroxysteroid dehydrogenase-isomerase.
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有证据表明相同的结构基因编码睾丸和肾上腺 3 β-羟基类固醇脱氢酶异构酶。
DOI：
10.1007/bf00498961
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发表时间：
1987
影响因子：
2.4
通讯作者：
Payne,AH
中科院分区：
文献类型：
--
作者：
Stalvey,JR;Meisler,MH;Payne,AH
研究了3β-羟基类固醇脱氢酶异构酶（3βHSD）活性在几种自交系和野生小鼠睾丸和肾上腺中的热稳定性。在野生Brno小鼠中检测到3βHSD的耐热性变异。3βHSD对C57BL/6J小鼠的睾丸和肾上腺的热稳定性（t1/2）约为7 min，而Brno小鼠的两种组织的热稳定性均为4 min。6种小鼠睾丸和肾上腺3βHSD热稳定性的比较表明，3βHSD的t1/2在两种组织中具有相关性，可分为耐热性和耐热性两种不同类型。相比之下，3βHSD活性的定量变异在两种组织中不相关。这些数据与睾丸和肾上腺3βHSD由相同结构基因编码的假设一致，但睾丸和肾上腺中3βHSD活性的表达是独立控制的。
Thermostability of 3β-hydroxysteroid dehydrogenase-isomerase (3βHSD) activity was examined in testes and adrenal glands from several inbred lines and feral mice. A thermolabile varant of 3βHSD was detected in the feral Brno mice. The thermostability (t1/2) of 3βHSD was approximately 7 min for both testes and adrenal glands from C57BL/6J mice, compared with 4 min for both tissues from Brno mice. Comparison of testicular and adrenal 3βHSD thermostability in six kinds of mice indicated that thet1/2of 3βHSD was correlated in the two tissues and could be classified into two distinct types, thermolabile and thermostable. In contrast, quantitative variants in 3βHSD activity were not correlated in the two tissues. These data are consistent with the hypothesis that testicular and adrenal 3βHSD is encoded by the same structural gene but that expression of 3βHSD activity is independently controlled in testes and adrenal glands.