Plasma membrane changes in the rat corpus luteum induced by oxygen radical generation.
Plasma membrane changes in the rat corpus luteum induced by oxygen radical generation.
复制标题
氧自由基的产生引起大鼠黄体质膜变化。
DOI：
10.1210/endo.133.2.8344194
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发表时间：
1993
期刊：
影响因子：
4.8
通讯作者：
J. Carlson
中科院分区：
文献类型：
--
作者：
X. Wu;K. Yao;J. Carlson
在大鼠黄体(CL)模型上研究了黄体溶解作用的机制。本研究的重点是质膜发生的变化。先前用大鼠黄体细胞进行的实验表明，黄嘌呤氧化酶在体外产生的超氧阴离子自由基破坏了黄体生成素刺激的cAMP的产生和孕酮的分泌，这与黄体溶解激素前列腺素F2α的作用类似。在本研究中，我们观察到黄嘌呤氧化酶处理的CL质膜样品的流动性显著降低，在前列腺素F2α诱导的黄体溶解过程中也发生了这种情况。这种流动性变化可被过氧化氢酶、磷脂酶-A活性抑制剂溴代苯乙酰、吲哚美辛和自由基清除剂所阻断，并被膜上游离脂肪酸的去除所逆转。此外，黄嘌呤氧化酶处理引起磷脂分解，中性脂形成，无机过氧化产物大量产生，过氧化脂质水平持续升高。这些结果表明，自由基的产生引起了CL细胞质膜的几个变化，这些变化增加了磷脂破坏的可能性，这些变化可能参与了黄体溶解时抑制黄体生成素刺激类固醇生成的机制。
The luteolytic mechanism was investigated in rat corpora lutea (CL). This study focused on the changes that occur in the plasma membrane. Previous experiments with rat luteal cells indicated that in vitro generation of superoxide radicals by xanthine oxidase disrupted LH-stimulated cAMP production and progesterone secretion similar to the effect of prostaglandin F2 alpha, the luteolytic hormone. In the present study, we observed that xanthine oxidase treatment of plasma membrane samples from CL caused a large decrease in fluidity, which also occurs during prostaglandin F2 alpha-induced luteolysis. This fluidity change was blocked by catalase, bromophenacyl bromide, an inhibitor of phospholipase-A activity, indomethacin, and free radical scavengers, and it was reversed by removal of FFA from the membrane. In addition, xanthine oxidase treatment caused phospholipid breakdown, formation of neutral lipids, a burst of inorganic peroxides, and a sustained rise in the level of lipid peroxides. These results indicate that free radical generation causes several changes that disrupt the plasma membrane of CL cells, and they raise the possibility that phospholipid breakdown could be involved in the mechanism that inhibits LH stimulation of steroidogenesis during luteolysis.