PROTEIN PHOSPHATASE-1 ACTIVITY IN DROSOPHILA MUTANTS WITH ABNORMALITIES IN MITOSIS AND CHROMOSOME CONDENSATION
PROTEIN PHOSPHATASE-1 ACTIVITY IN DROSOPHILA MUTANTS WITH ABNORMALITIES IN MITOSIS AND CHROMOSOME CONDENSATION
复制标题
DOI：
10.1016/0014-5793(90)81434-p
复制
发表时间：
1990-11-26
期刊：
影响因子：
3.5
通讯作者：
COHEN, PTW
中科院分区：
文献类型：
--
作者：
DOMBRADI, V;AXTON, JM;COHEN, PTW
已对编码蛋白磷酸酶1（PPI）的ADrosophilagene进行了测序，并分析了该位点（87 B）的致死突变。两个突变体（ck19e211和ck19hs46），破坏有丝分裂，缺乏87 B同工酶，仅表达野生型PPI活性的约20%。在k19e11突变体中，该基因的启动子区缺失。第三个突变体（ck19e078）显示出位置效应杂色的抑制，但对有丝分裂几乎没有影响，具有野生型PPI活性的约35%。结果表明，PPI87B同工酶不仅参与有丝分裂过程，而且参与间期染色体凝聚的调节。
ADrosophilagene encoding a protein phosphatase 1(PPI) has been sequenced, and lethal mutations in this locus (87B) analysed. Two mutants (ck19e211andck19hs46), which disrupt mitosis, lack the 87B isoenzyme and express only ∼20% of wild type PPI activity. The Promoter region of the gene is deleted in theck19e11mutant. A third mutant (ck19e078), which shows suppression of position effect variegation, but has little effect on mitosis, possesses ∼35% of wild type PPI activity. The results indicate that the PPI 87B isoenzyme is involved in regulation of chromosome condensation at interphase as well as mitosis.