The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides
The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides
复制标题
DOI：
10.1016/j.biomaterials.2004.02.012
复制
发表时间：
2005-01-01
期刊：
影响因子：
14
通讯作者：
Davies, N
中科院分区：
文献类型：
--
作者：
Fittkau, MH;Zilla, P;Davies, N
评估了仿生肽YIGSR、PHSRN和RGD选择性影响人微血管内皮细胞（MVEC）和血管平滑肌细胞（HVSMC）粘附和迁移的能力。通过在肽修饰表面上迁移的单细胞的延时视频显微镜来量化细胞迁移率。用 YIGSR 或 PHSRN 修饰的聚乙二醇 (PEG) 水凝胶仅允许有限的粘附，并且 MVEC 和 HVSMC 不会扩散。然而，当这些肽单独与 PEG 水凝胶中的强细胞结合肽 RGD 结合时，发现 YIGSR 肽比单独使用 RGD 的 MVEC 选择性增强 MVEC 的迁移 25%（p
The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25% over that of MVEC on RGD alone (p