A method for extracting ferruginous bodies from sputum and pulmonary tissue.

A method for extracting ferruginous bodies from sputum and pulmonary tissue.
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一种从痰液和肺组织中提取铁质体的方法。

DOI:
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发表时间:
1972
影响因子:
3.5
通讯作者:
Bernard Naylor
Bernard Naylor
中科院分区:
医学4区
文献类型:
--
作者:
M. Jane Smith;Bernard Naylor

文献摘要

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描述了一种从痰和固定或未固定的肺中提取铁质体的方法。其原理是在家用洗衣漂白剂(5.25%次氯酸钠)中完全消化痰液或组织,并在膜过滤器上收集固体残留物。这种方法几乎可以从标本中提取光学显微镜可见的所有铁质物体。通过这种方法,在连续尸检的100名16岁及以上患者的肺组织中均发现了铁锈体。在一个案例中,发现铁质体的浓度约为每克 750,000 个。湿固定的肺组织。
A method for extracting ferruginous bodies from sputum and fixed or unfixed lung is described. The principle is to digest the sputum or tissue completely in domestic laundry bleach (5.25% sodium hypochlorite) and to collect the solid residue on a membrane filter. This method allows one to extract from a specimen virtually every ferruginous body that is visible with the light microscope. With this method, ferruginous bodies were found in pulmonary tissue from each of 100 consecutive necropsies of patients 16 years old and over. In one case, ferruginous bodies were found in a concentration of about 750,000 per Gm. of wet fixed pulmonary tissue.