Raman Spectroscopy of Bacterial Species and Strains Cultivated under Reproducible Conditions
Raman Spectroscopy of Bacterial Species and Strains Cultivated under Reproducible Conditions
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DOI：
10.1155/2012/540490
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Telle, Helmut H.
中科院分区：
文献类型：
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作者：
Almarashi, Jamal F. M.;Kapel, Natalia;Telle, Helmut H.
在微生物实验室处理受感染的临床样本时，快速和可重复地区分细菌病原体是一个明确的目标。在这项研究中，使用雷尼绍VIA拉曼显微镜系统采集了包括表皮葡萄球菌(1457和9142)和大肠杆菌(K12和Top 10)在内的四种细菌的至少30个菌落的拉曼光谱。基于主成分的分析表明，即使菌株分化(例如，1457对9142或K12对Top10)也是可能的。
Rapid and reproducible discrimination between bacterial pathogens is a clear goal in microbiological laboratories when processing infected clinical samples. In this study Raman spectra were taken from at least 30 colonies of four strains of bacteria including Staphylococcus epidermidis (1457 and 9142) and Escherichia coli (K12 and Top 10) using the Renishaw in Via Raman microscope system. Analysis based on principal components suggests that even strain differentiation (e. g., 1457 versus 9142 or K12 versus Top10) is possible.