An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests
An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests
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DOI：
10.1016/j.bios.2020.112651
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发表时间：
2020-12-01
影响因子：
12.6
通讯作者：
Lu, Meng
中科院分区：
文献类型：
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作者：
Liu, Mingdian;Zhao, Yuxin;Lu, Meng
纸基传感器可用于开发低成本、一次性和快速的检测方法，用于检测各种各样的分析物。我们报告了一种用于即时（POC）核酸扩增测试的纸基传感器系统，该系统可以定量检测来自不同病原体的多个基因。POC系统结合了纸质传感器芯片和便携式仪器，构建在物联网（IoT）平台上。纸基传感器提供试剂储存、样品运输和核酸扩增的功能。物联网仪器使用Arduino微控制器来控制温度，收集荧光图像，并通过WiFi网络将数据存储在云存储中。设计了一种紧凑型荧光读取器，用于在环介导的等温扩增反应期间实时测量扩增子的荧光图像。实时检测能力使靶基因的定量分析成为可能。结果表明，该纸基传感器可在低浓度（2 × 10（3）copies/mu L）下，对大肠杆菌和空肠弯曲菌基因组DNA中的多个基因进行区分。负担得起的仪器，结合一次性纸传感器芯片，将有一个很大的潜力POC检测病原体。
Paper-based sensors can be exploited to develop low-cost, disposable, and rapid assays for the detection of a large variety of analytes. We report a paper-based sensor system for a point-of-care (POC) nucleic acid amplification test that can quantitatively detect multiple genes from different pathogens. The POC system combines a paper sensor chip and a portable instrument, which is built on an Internet of Things (IoT) platform. The paper-based sensor provides the functions of reagent storage, sample transportation, and nucleic acid amplification. The IoT instrument uses an Arduino microcontroller to control temperature, collect fluorescence images, and store the data in cloud storage via a WiFi network. A compact fluorescence reader was designed to measure fluorescence images of the amplicons during a loop-mediated isothermal amplification reaction in real-time. The real-time detection capability enables the quantitative analysis of target genes. The results show that the paper-based sensor cam distinguish multiple genes of the genomic DNA extracted from Escherichia coli and Campylo-bacter jejuni, with the concentration as low as 2 x 10(3) copies/mu L. The affordable instrument, in conjunction with the disposable paper sensor chip, would have a great potential for POC detections of pathogens.