Functional role of Forskolin and PD166285 in the development of denuded mouse oocytes
Functional role of Forskolin and PD166285 in the development of denuded mouse oocytes
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Forskolin 和 PD166285 在裸鼠卵母细胞发育中的功能作用
DOI：
10.5713/ajas.17.0441
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
曹鸿国
中科院分区：
文献类型：
--
作者：
曹鸿国
目的研究环磷酸腺苷（cAMP）和成熟促进因子（MPF）在哺乳动物卵母细胞成熟过程中的作用。本研究的目的是通过调控cAMP和MPF来诱导小鼠裸卵母细胞产生子代。方法采用小鼠生发泡期的DOs，在体外成熟过程中分别加入Forskolin和PD 166285调节DOs中cAMP和MPF的水平。结果50 μM的Forskolin作用3 h，2.5 μM的PD 166285作用21 h，可提高孤雌激活后的发育能力，孤雌激活后的成熟率为76.71%± 4.11%，囊胚率为18.33%±4.44%。体外受精成功，卵裂率为17.02%±5.82%，囊胚率为5.65%± 3.10%。此外，2-细胞体外受精胚胎产生4个正常的活仔（4/34）。结论Forskolin和PD 166285联合应用可诱导Dos完成减数分裂过程并产生正常子代。
Objective cAMP and mature promoting factor (MPF) play critical roles during the maturation of mammalian oocytes. The aim of this study was to produce the offspring from denuded oocytes (DOs) in mice by regulating cAMP and MPF. Methods In this study, we used DOs at the germinal vesicle (GV) stage in mice and regulated levels of cAMP and MPF in DOs by adding Forskolin and PD166285 during in vitro maturation without follicle stimulating hormone and luteinizing hormone, respectively. Results Combined use of 50 μM Forskolin for 3 h and 2.5 μM PD166285 for additional 21 h enhanced the developmental competence of DOs, maturation rate of DOs was 76.71%± 4.11%, blastocyst rate was 18.33%±4.44% after parthenogenetic activation (PA). The DOs could successfully be fertilized with sperm in vitro, cleavage rate was 17.02%±5.82% and blastocyst rate was 5.65%±3.10%. Besides, 2-cell in vitro fertilization embryos from DOs produced 4 normal live offspring (4/34). Conclusion The results confirmed that the combination of Forskolin and PD166285 can induce DOs to complete meiosis process and produce normal offspring.
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影响因子：
2.5
作者：
Lambert, Charles C.
通讯作者：
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影响因子：
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作者：
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A. Schmitt;A. Nebreda
DOI：
--
发表时间：
2005
期刊：
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影响因子：
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