Osteoclasts are recruited to the subchondral bone in naturally occurring post-traumatic equine carpal osteoarthritis and may contribute to cartilage degradation
Osteoclasts are recruited to the subchondral bone in naturally occurring post-traumatic equine carpal osteoarthritis and may contribute to cartilage degradation
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DOI：
10.1016/j.joca.2015.10.008
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发表时间：
2016-03-01
影响因子：
7
通讯作者：
Laverty, S.
中科院分区：
文献类型：
--
作者：
Bertuglia, A.;Lacourt, M.;Laverty, S.
破骨细胞在骨关节炎（OA）的骨软骨变性的作用很少被调查在自发性疾病或动物模型OA.Objective：目前的研究的目的是探讨破骨细胞的密度和位置在创伤后OA（PTOA）和对照标本从racemors.Method：核心收获从一个网站在马第三腕骨，经历重复，高强度的负荷。对组织学和免疫组织化学（组织蛋白酶K和核因子κ-β配体受体激活剂（RANKL））染色切片进行评分（整体和次区域），并计算破骨细胞密度。软骨组织学评分与破骨细胞密度和RANKL评分进行比较。结果：PTOA标本中破骨细胞密度较高，且优先分布于软骨下骨板。RANKL评分与软骨退变评分、破骨细胞密度呈正相关。透明关节软骨RANKL评分和破骨细胞密度之间的关系比软骨下骨RANKL评分更强，表明软骨RANKL可能在招募破骨细胞中发挥作用。RANKL评分在关节钙化软骨与微裂纹的数量也表明，破骨细胞招募RANKL可能有助于钙化软骨变性PTOA。结论：我们的研究结果支持这一假设，即破骨细胞招募过程中自发马腕PTOA软骨RANKL，有助于钙化软骨微裂纹和局灶性软骨下骨丢失。(C)2015国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
The role of osteoclasts in osteochondral degeneration in osteoarthritis (OA) has rarely been investigated in spontaneous disease or animal models of OA.Objective: The objectives of the current study were to investigate osteoclast density and location in post-traumatic OA (PTOA) and control specimens from racehorses.Method: Cores were harvested from a site in the equine third carpal bone, that undergoes repetitive, high intensity loading. Histological and immunohistochemical (Cathepsin K and Receptor-activator of Nuclear Factor kappa-beta ligand (RANKL)) stained sections were scored (global and subregional) and the osteoclast density calculated. The cartilage histological scores were compared with osteoclast density and RANKL scores.Results: There was a greater density of osteoclasts in PTOA samples and they were preferentially located in the subchondral bone plate. RANKL scores positively correlated to the scores of cartilage degeneration and the osteoclast density. The relationship between hyaline articular cartilage RANKL score and osteoclast density was stronger than that of the subchondral bone RANKL score suggesting that cartilage RANKL may have a role in recruiting osteoclasts. The RANKL score in the articular calcified cartilage correlated with the number of microcracks also suggesting that osteoclasts recruited by RANKL may contribute to calcified cartilage degeneration in PTOA.Conclusion: Our results support the hypothesis that osteoclasts are recruited during the progression of spontaneous equine carpal PTOA by cartilage RANKL, contributing to calcified cartilage microcracks and focal subchondral bone loss. (C) 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.