Nucleoplasmic calcium is required for cell proliferation
Nucleoplasmic calcium is required for cell proliferation
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DOI：
10.1074/jbc.m700490200
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发表时间：
2007-06-08
影响因子：
4.8
通讯作者：
Nathanson, Michael H.
中科院分区：
文献类型：
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作者：
Rodrigues, Michele A.;Gomes, Dawidson A.;Nathanson, Michael H.
钙信号调节细胞增殖，但这些信号的时空特异性尚不清楚。在这里，我们使用核质或细胞质钙离子的选择性缓冲液来确定细胞的增殖依赖于细胞核内的钙信号，而不是细胞质中的钙信号。核钙信号刺激细胞生长，而不是抑制细胞凋亡，特别是允许细胞通过早期阶段。核内而不是胞浆内钙离子信号的选择性缓冲也会损害体内肿瘤的生长。这些发现揭示了核质钙信号的主要生理和潜在的病理生理学作用，并表明这些信息可以用来设计新的治疗策略来调节细胞异常生长的条件。
Ca2+ signals regulate cell proliferation, but the spatial and temporal specificity of these signals is unknown. Here we use selective buffers of nucleoplasmic or cytoplasmic Ca2+ to determine that cell proliferation depends upon Ca2+ signals within the nucleus rather than in the cytoplasm. Nuclear Ca2+ signals stimulate cell growth rather than inhibit apoptosis and specifically permit cells to advance through early prophase. Selective buffering of nuclear but not cytoplasmic Ca2+ signals also impairs growth of tumors in vivo. These findings reveal a major physiological and potential pathophysiological role for nucleoplasmic Ca2+ signals and suggest that this information can be used to design novel therapeutic strategies to regulate conditions of abnormal cell growth.