Development of microfluidic device for electrical/physical characterization of single cell
Development of microfluidic device for electrical/physical characterization of single cell
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DOI：
10.1109/jmems.2005.863738
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发表时间：
2006-04-01
影响因子：
2.7
通讯作者：
Kim, B
中科院分区：
文献类型：
--
作者：
Cho, YH;Yamamoto, T;Kim, B
提出了一种具有流动细胞微通道和双微悬臂梁阵列的新型装置，用于测量单个细胞的电阻抗。演示了制造过程，并成功制造了双微悬臂梁阵列。在我们的研究中，我们测量了 1 Hz 至 10 MHz 频率范围内正常和异常红细胞的电阻抗。从正常红细胞和异常红细胞的电阻抗实验中可以看出，正常红细胞和异常红细胞之间的电阻抗在大小和相移方面存在显着差异。在本文中，我们证明可以通过电阻抗测量将正常细胞与异常细胞分开。因此，预计该技术的适用性可用于细胞研究，例如细胞分选、计数或膜生物物理表征。
A novel device with microchannels for flowing cells and twin microcantilever arrays for measuring the electrical impedance of a single cell is proposed. The fabrication process is demonstrated and the twin microcantilever arrays have been successfully fabricated. In our research, we measured the electrical impedance for normal and abnormal red blood cell over the frequency range from 1 Hz to 10 MHz. From the electrical impedance experiment of normal and abnormal red blood cell, it was examined that the electrical impedance between normal and abnormal red blood cells was significantly different in magnitude and phase shift. In this paper, we show that the normal cell can be taken apart from the abnormal cell by electrical impedance measurement. Therefore, it is expected that the applicability of this technology can be used in cellular studies such as cell sorting, counting or membrane biophysical characterization.