Electro-acupuncture at ST37 and ST25 induce different effects on colonic motility via the enteric nervous system by affecting excitatory and inhibitory neurons
Electro-acupuncture at ST37 and ST25 induce different effects on colonic motility via the enteric nervous system by affecting excitatory and inhibitory neurons
复制标题
电针 ST37 和 ST25 通过影响兴奋性和抑制性神经元，通过肠神经系统对结肠运动产生不同的影响
DOI：
10.1111/nmo.13318
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发表时间：
2018-07-01
影响因子：
3.5
通讯作者：
Xu, B.
中科院分区：
文献类型：
--
作者：
Liang, C.;Wang, K. -Y.;Xu, B.
研究背景肠神经系统（enteric nervous system，ENS）在胃肠运动中的重要作用，提示ENS可能通过调节电针对胃肠功能的影响而介导电针对便秘的治疗作用。评估排便时间和粪便（形状和湿重和干重）。在ST 37或ST 25的EA对结肠直肠运动和近端结肠运动的影响进行了评估，使用充满水的气球。蛋白基因产物9.5（PGP9.5）、胆碱能神经元标志物乙酰转移酶（ChAT）和抗胆碱能神经元标志物一氧化氮合酶（nNOS）的表达通过免疫组织化学、实时定量聚合酶链反应（qPCR）和蛋白质印迹分析（western blot analysis）检测。在近端结肠中，在ST 37电针后PGP9.5的表达恢复正常，而ST 25电针没有这种影响。此外，qPCR和western blot分析显示，ST37可下调nNOS的表达，上调ChAT的表达至正常水平，而ST25仅下调nNOS的表达至正常水平。结论与推论电针特定穴位可通过改变ENS和不同程度地影响兴奋性和抑制性神经元来改善便秘时的肠动力，恢复收缩和放松肌肉之间的协调，并与中枢神经系统和外周神经通路协同工作。
BackgroundOn the basis of the importance of the enteric nervous system (ENS) in gastrointestinal motility, we hypothesized that the ENS may mediate the therapeutic efficacy of electro-acupuncture (EA) in constipation by regulating the mechanisms underlying the effects of EA on gastrointestinal function.MethodsModel mice with constipation were generated by gastric instillation of 0-4 degrees C normal saline. Defecation time and stool (form and wet and dry weight) were assessed. The effect of EA at ST37 or ST25 on colorectal motility and proximal colonic motility was assessed using a water-filled balloon. The expression of protein gene product 9.5 (PGP9.5), the cholinergic neuron marker acetyltransferase (ChAT) and the anticholinergic neuron marker nitric oxide synthase (nNOS) was detected by immunohistochemistry, real-time quantitative polymerase chain reaction (qPCR) and western blot analysis.Key ResultsST37 and ST25 improved colorectal pressure; however, ST37 but not ST25 improved proximal colonic pressure. In the proximal colon, the expression of PGP9.5 returned to normal after EA at ST 37, while EA at ST25 did not have this effect. In addition, qPCR and western blot analysis showed that ST37 could downregulate the expression of nNOS and upregulate the expression of ChAT to normal levels, while ST25 could only downregulate the expression of nNOS to normal levels.Conclusions and InferencesElectro-acupuncture at specific acupoints can improve intestinal motility in constipation by altering the ENS and differentially affecting excitatory and inhibitory neurons, restoring the coordination between contraction and relaxation muscles, and working in concert with the central nervous system and peripheral neural pathways.