Monoallelic expresion of the odourant receptor gene and axonal projection of olfactory sensory neurones
Monoallelic expresion of the odourant receptor gene and axonal projection of olfactory sensory neurones
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DOI：
10.1046/j.1365-2443.2001.00398.x
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发表时间：
2001-01-01
期刊：
影响因子：
2.1
通讯作者：
Sakano, H
中科院分区：
文献类型：
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作者：
Ishii, T;Serizawa, S;Sakano, H
背景：我们先前已经产生了转基因小鼠携带鼠气味受体基因，MOR28，标记lacZ。在该动物中，内源性M0R 28用GFP不同地标记。结果发现，转基因和内源性MOR28基因的表达在一个相互排斥的方式和两套嗅觉感觉神经元（OSNs），每个表达无论是转基因或内源性MOR28，项目的轴突分离glomeruli.Results：我们的荧光原位杂交（FISH）显示，MOR28的两个内源性等位基因也相互排斥的转录激活。因此，我们研究了OSN的两个子集的投影中是否存在任何分离：一个集合表达父系等位基因，另一个表达母系等位基因。结果发现，两个等位基因的OSNs共享相同的肾小球，他们的投影，但投影目标被隔离内的肾小球structure.Conclusion：两个子集的神经元表达的转基因或内源性MOR28的目标，其轴突到两个单独的肾小球的基础上的遗传背景，性质的标签，和染色体的位置的差异。相反，表达母本或父本等位基因的神经元共享相同的肾小球，但倾向于靶向肾小球结构内的隔离区域。随着两个等位基因间遗传背景差异的增大，分离现象更加突出。
Background: We have previously generated transgenic mice carrying the murine odourant receptor gene, MOR28, tagged with lacZ. In this animal, the endogenous MOR28 is differently tagged with GFP. It was found that the transgenic and endogenous MOR28 genes are expressed in a mutually exclusive manner and that the two sets of olfactory sensory neurones (OSNs), each expressing either the transgenic or the endogenous MOR28, project their axons to separate glomeruli.Results: Our fluorescent in situ hybridization (FISH) revealed that the two endogenous alleles of MOR28 are also mutually excluded for their transcriptional activation. Therefore, we studied whether there would be any segregation in the projection of the two subsets of OSNs: one set expressing the paternal and the other expressing the maternal allele. It was found that the OSNs for both alleles shared the same glomerulus for their projection, but the projection targets were segregated within the glomerular structure.Conclusion: Two subsets of neurones expressing either the transgenic or the endogenous MOR28 target their axons to two separate glomeruli based on the differences in the genetic backgrounds, nature of tagging, and chromosomal locations. In contrast, neurones expressing a maternal or paternal allele share the same glomeruli, but tend to target to segregated areas within the glomerular structure. The segregation was more prominent with increased differences in the genetic background between the two alleles.