Combining Untargeted and Targeted Proteomic Strategies for Discrimination and Quantification of Cashmere Fibers.
Combining Untargeted and Targeted Proteomic Strategies for Discrimination and Quantification of Cashmere Fibers.
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结合非靶向和靶向蛋白质组策略来辨别和定量羊绒纤维
DOI：
10.1371/journal.pone.0147044
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Shui W
中科院分区：
文献类型：
--
作者：
Li S;Zhang Y;Wang J;Yang Y;Miao C;Guo Y;Zhang Z;Cao Q;Shui W
羊绒因其稀缺性和极高的经济价值而被视为特种和奢侈纤维。对于纤维质量评估，由于羊绒纤维与牦牛或羊毛纤维高度相似的物理外观和大量的蛋白质序列同源性，因此在技术上区分和定量羊绒纤维是非常具有挑战性的。为了解决这个问题，我们提出了一个工作流程结合非靶向和有针对性的蛋白质组学策略选择，验证和量化生物标志物的羊绒纺织品认证。首次应用非靶向蛋白质组学研究，分别从羊绒、羊毛和牦牛绒中鉴定出174、157和156种蛋白质。经过不同水平的标记选择后，发现肽对纤维种类的鉴别具有比蛋白质高得多的选择性。随后，平行反应监测（PRM）的方法，开发了10个选定的肽标记。基于PRM的肽标记物靶向分析能够准确测定不同纤维混合物中的纤维种类和羊绒百分比。此外，这些肽制造商的集体使用使我们能够区分和量化商业成品织物中的羊绒纤维，这些织物经过了重化学处理。使用我们的蛋白质组学方法测量织物样品中的羊绒比例与传统光学显微镜的结果非常一致，但我们的方法可以更容易地标准化，成为评估纤维和纺织品真实性的客观和可靠的检测方法。我们预计，在我们的研究中提出的蛋白质组学策略可能会进一步涉及发现其他产品的质量性状标记含有高度同源的蛋白质组。
Cashmere is regarded as a specialty and luxury fiber due to its scarcity and high economic value. For fiber quality assessment, it is technically very challenging to distinguish and quantify the cashmere fiber from yak or wool fibers because of their highly similar physical appearance and substantial protein sequence homology. To address this issue, we propose a workflow combining untargeted and targeted proteomics strategies for selecting, verifying and quantifying biomarkers for cashmere textile authentication. Untargeted proteomic surveys were first applied to identify 174, 157, and 156 proteins from cashmere, wool and yak fibers, respectively. After marker selection at different levels, peptides turned out to afford much higher selectivity than proteins for fiber species discrimination. Subsequently, parallel reaction monitoring (PRM) methods were developed for ten selected peptide markers. The PRM-based targeted analysis of peptide markers enabled accurate determination of fiber species and cashmere percentages in different fiber mixtures. Furthermore, collective use of these peptide makers allowed us to discriminate and quantify cashmere fibers in commercial finished fabrics that have undergone heavy chemical treatments. Cashmere proportion measurement in fabric samples using our proteomic approach was in good agreement with results from traditional light microscopy, yet our method can be more readily standardized to become an objective and robust assay for assessing authenticity of fibers and textiles. We anticipate that the proteomic strategies presented in our study could be further implicated in discovery of quality trait markers for other products containing highly homologous proteomes.