PROLIFERATING CELL NUCLEAR ANTIGEN AND P21 ARE COMPONENTS OF MULTIPLE CELL-CYCLE KINASE COMPLEXES
PROLIFERATING CELL NUCLEAR ANTIGEN AND P21 ARE COMPONENTS OF MULTIPLE CELL-CYCLE KINASE COMPLEXES
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DOI：
10.1091/mbc.4.9.897
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发表时间：
1993-09-01
影响因子：
3.3
通讯作者：
BEACH, D
中科院分区：
文献类型：
--
作者：
HUI, Z;YUE, X;BEACH, D
我们最近发现，两种蛋白质，增殖细胞核抗原（PCNA）和p21，与细胞周期蛋白D。在这里，我们表明，PCNA和p21的共同组成部分的各种各样的细胞周期蛋白/细胞周期蛋白依赖性激酶复合物在非转化细胞。这些包括含有细胞周期蛋白A、细胞周期蛋白B和细胞周期蛋白D的激酶复合物，其与CDC 2、CDK 2、CDK 4或CDK 5相关。我们发现，PCNA和p21形成单独的四元复合物与每个细胞周期蛋白/CDK，这些四元复合物包含大量的，如果不是主要的，部分细胞周期激酶在异步生长的细胞。这些结果表明，PCNA和p21可能执行所有这些激酶的共同功能。
We have recently shown that two proteins, proliferating cell nuclear antigen (PCNA) and p21, are associated with cyclin D. Here we show that PCNA and p21 are common components of a wide variety of cyclin/cyclin-dependent kinase complexes in nontransformed cells. These include kinase complexes containing cyclin A, cyclin B, and cyclin D, associated either with CDC2, CDK2, CDK4, or CDK5. We show that PCNA and p21 form separate quaternary complex with each cyclin/CDK and that these quaternary complexes contain a substantial, if not major, fraction of the cell cycle kinases in asynchronously growing cells. These results suggest that PCNA and p21 may perform a common function for all these kinases.