Quantitative studies on concanavalin A-induced, TCGF-reactive T cells. I. Correlation between proliferation and lectin-dependent cytolytic activity.
Quantitative studies on concanavalin A-induced, TCGF-reactive T cells. I. Correlation between proliferation and lectin-dependent cytolytic activity.
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对刀豆球蛋白 A 诱导的 TCGF 反应性 T 细胞的定量研究。
DOI：
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发表时间：
1981
影响因子：
4.4
通讯作者：
Antonio Coutinho
中科院分区：
文献类型：
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作者：
E. Larsson;K. Lindahl;Jean Langhorne;Antonio Coutinho
采用有限稀释法测定可被Con A和TCGF诱导增殖和杀伤的T细胞频率。5个尼龙毛纯化脾细胞和7个正常淋巴结细胞中分别有1个增殖细胞。凝集素依赖性杀伤细胞前体在相同细胞群中的频率分别为1/5和1/9。即使在每个培养物接种的T细胞数量有限的情况下，也很少有培养物增殖而不显示细胞毒性活性。因此，在这些条件下，Con A和TCGF优先地（如果不是排他地）激活在PHA存在下将杀死的T细胞。该实验系统可用于估计由Con A激活并在TCGF中扩增的特异性CTL前体的库。
A limiting dilution assay was used to determine the frequencies of T cells that could be induced to proliferate and kill by Con A and TCGF. The frequency of proliferating cells was 1 in 5 nylon wool-purified spleen cells and 1 in 7 normal lymph node cells. The frequencies in the same cell populations of lectin-dependent killer cell precursors were 1 in 5 and 1 in 9, respectively. Even at limiting numbers of T cells seeded per culture, very few cultures proliferated without showing cytotoxic activity. Thus, under these conditions Con A and TCGF, preferentially if not exclusively, activate T cells that will kill in the presence of PHA. This experimental system can be used for estimating the repertoires of specific CTL-precursors activated by Con A and expanded in TCGF.